Development of Particle Packed Bed Type Chromatography Using Dielectrophoresis

Yusuke Umezawa; Okihisa Kobayashi; Syoji Kanai; Masaru Hakoda

doi:10.4028/www.scientific.net/KEM.534.88

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HomeKey Engineering MaterialsKey Engineering Materials Vol. 534Development of Particle Packed Bed Type...

Development of Particle Packed Bed Type Chromatography Using Dielectrophoresis

Abstract:

In this study, we analyzed differences in cell species using dielectrophoresis (DEP) and propose a particle packed bed type chromatography device using DEP. Mouse-hybridoma 3-2H3 cells were used as the sample cells. The 3-2H3 cell suspension was fed pulse-wise to a carrier flow and the sample was measured by an absorption spectrometer. The analytical characteristic of chromatography using DEP was evaluated by the retention time difference. We examined the effects of the diameter of packed particles on the retention time difference. The retention time difference in the case of the packed particles of 100 μm diameter was larger than that of 500 μm, which suggests that packed particles of smaller diameter are suitable for analysis using DEP chromatography.

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Periodical:

Key Engineering Materials (Volume 534)

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88-92

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.534.88

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Online since:

January 2013

Authors:

Yusuke Umezawa, Okihisa Kobayashi, Syoji Kanai, Masaru Hakoda

Keywords:

AC Electric Field, Analysis Equipment, DEP Chromatography, Dielectrophoresis

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References

[1] J. S. Crance and H.A. Pohl: J. Electrochem. Soc. Vol. 115 (1968), p.175.

Google Scholar

[2] Y. Hirota, M. Hakoda and Y. Wakizaka: Bioprocess and Biosystem Eng. Vol. 39 (2003), p.1514.

Google Scholar

[3] M. Hakoda, Y. Wakizaka, S. Mii and N. Kitajima: J. Inst. Electrostat. Japan. Vol. 29 (2005), p.8.

Google Scholar

[4] M. Hakoda, Y. Wakizaka, Y. Hirota and N. Kitajima: Biotechnology Progress. Vol. 26 (2010) p.1062.

Google Scholar

[5] M. Hakoda, M. Toshinai and Y. Wakizaka: J. Inst. Electrostat. Japan. Vol. 30 (2005) p.140.

Google Scholar

[6] P. R. C. Gascoyne, J. Noshari, T. J. Anderson, F. F. Becker: Electrophoresis Vol. 30 (2009), p.1388.

Google Scholar

[7] Y. Hirota and M. Hakoda: Key Engineering Materials Vol. 459 (2011) p.84.

Google Scholar

[8] X-B Wang, J. Vykoukal, F. F. Becker, and P. R. C. Gascoyne: Biophysical Journal Vol. 74 (1998), p.2689.

Google Scholar

[9] J. Yang, Y. Huang, X-B Wang, F. F. Becker, and P. R. C. Gascoyne: Biophysical Journal Vol. 78 (2000), p.2680.

Google Scholar

[10] J. Suehiro, G. Zhou, M. Imamura, M. Hara: Industry Applications, IEEE Transactions Vol. 39 (2003), p.1514.

Google Scholar

[11] C. Iliescu, G. Xu, F. C. Loe, P. L. Ong, F. E.H. Tay: Electrophoresis Vol. 28 (2008), p.1107.

Google Scholar