Two Benches PDMS Free Flow Electrophoresis Chip

Wei Wang; Li Tian; Xiao Wei Han; Lin Zhao; Guo Hua Ye; Hai Feng Zhang; Xiao Wei Liu

doi:10.4028/www.scientific.net/KEM.609-610.769

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HomeKey Engineering MaterialsKey Engineering Materials Vols. 609-610Two Benches PDMS Free Flow Electrophoresis Chip

Two Benches PDMS Free Flow Electrophoresis Chip

Abstract:

This paper puts forward a kind of two benches structure of the glass-PDMS free flow electrophoresis chip. We use die casting method to produce microstructure, and use PDMS as its structure layer. The mold is made in tape-PMMA by micro cementing technology. During the progress of PDMS curing, gold wires are directly integrated in the electrode groove to form the chips electrodes. PDMS and glass are sealed in natural bonding method. Chips glass surface of separation chamber realizes the modification through sticking polyimide tape. Visual technology is conducted for chip electrophoresis separation. The sample is the mixture of methyl green and Rhodamine B. The separation effect of methyl green and Rhodamine B is obvious when the electric field is 31 V/cm. the "two benches" between the electrode groove and the separation chamber block the electrolytic bubbles into the separation chamber. When the separation chamber height is 70 μm and the electric field is 50 V/cm, the air bubbles can be glidingly discharged from electrode groove. The samples steady separation time is more than 2 hours, and this free flow electrophoresis chip realizes continuous work.

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Periodical:

Key Engineering Materials (Volumes 609-610)

Pages:

769-774

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.609-610.769

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Online since:

April 2014

Authors:

Wei Wang*, Li Tian, Xiao Wei Han, Lin Zhao, Guo Hua Ye, Hai Feng Zhang, Xiao Wei Liu

Keywords:

Free Flow Electrophoresis Chip, Free Flow Electrophoresis Separation, Micro Total Analysis System

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* - Corresponding Author

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