Yeast Cells Immobilization in Microfluidic Channels Based on a Self-Polymerized Nano-Film of Poly(Dopamine)

Abstract:

In this paper, an effective, simple and universal method for cell immobilization was developed. A self-polymerization nanofilm of poly (dopamine) was used to fix yeast cells in microfluidic channels. The surface morphology of the poly (dopamine) film was characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM) techniques. Water contact angles (WCA) was also used to characterize the surface property of the poly (dopamine) nanofilm. The WCA on the PDMS substrates rapidly decreased from 105° to 59.8° with an increase in poly (dopamine) coating time. The interfacial process of dopamine self-polymerization and the cell immobilization were measured in a label-free and real-time mode by a surface plasmon resonance (SPR) instrument. Finally the immobilized yeast cells were observed by using a light microscope. From the experimental results, the yeast cells can be easily immobilized on the microfluidic channels modified with the nanofilm of poly (dopamine), which will hold great potential for the immobilization, detection and further analysis of other suspension cells, such as blood cells.

• Periodical: Key Engineering Materials (Volumes 645-646)
• Pages: 1357-1362
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.645-646.1357
• Online since: May 2015
• Authors: Xing Chen*, Lu Lu Zhang, Jian Hai Sun, Hui Li, Da Fu Cui
• Keywords: Cell Immobilization, Micro Electro Mechanical Systems Technology (MEMS), Nano-Film, Self-Polymerization, Surface Plasmon Resonance (SPR)

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