Studies on Connexin 43, a Gap-Junction Protein, in P19 Embryonal Carcinoma Cells after Culture on an Apatite Fiber Scaffold

Nobuyuki Kanzawa; Hiroki Takano; Kei Yasuda; Masahiro Takahara; Mamoru Aizawa

doi:10.4028/www.scientific.net/KEM.696.230

Paper Titles

Mechanical Behavior of PCL Nanofibers
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Human Mesenchymal Stem Cells Behavior on Synthetic Coral Scaffold
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In Vivo Evaluation of Strontium-Containing Nanostructured Carbonated Hydroxyapatite
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In Vivo Evaluation of Zinc-Containing Nanostructured Carbonated Hydroxyapatite
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Studies on Connexin 43, a Gap-Junction Protein, in P19 Embryonal Carcinoma Cells after Culture on an Apatite Fiber Scaffold
p.230

Cytocompatiblity of Ceramic Nanoparticles to Various Types of Cells
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Hollow Shells Development and Characterization for Cells Carrying Purpose
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Bioceramic Drug Delivery System for Cancer Treatment and Regenerative Medicine
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Preparation of Carbonated Apatite Membrane as Metronidazole Delivery System for Periodontal Application
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 696Studies on Connexin 43, a Gap-Junction Protein, in...

Studies on Connexin 43, a Gap-Junction Protein, in P19 Embryonal Carcinoma Cells after Culture on an Apatite Fiber Scaffold

Abstract:

We previously showed enhanced osteoblast differentiation by culturing cells in apatite-fiber scaffold (AFS). The well-developed a-surface of the apatite fibers provides favorable structural features and chemical interactions with the cells, and the scaffold appears to affect cell differentiation. AFS was used here to study its utility for soft-tissue engineering. An embryonal carcinoma cell line, P19.CL6, was cultured in AFS, and the expression and phosphorylation of a gap-junction protein, connexin 43 (Cx43), during cell proliferation and differentiation was examined. We show that treatment with dimethyl sulfoxide appears to induce a change in the isoform composition of Cx43 under the control condition, but not in AFS. We also show that serum starvation induces the phosphorylation of Ser 368 of Cx43 only in functionally mature cells.

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Periodical:

Key Engineering Materials (Volume 696)

Pages:

230-233

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.696.230

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Online since:

May 2016

Authors:

Nobuyuki Kanzawa*, Hiroki Takano, Kei Yasuda, Masahiro Takahara, Mamoru Aizawa

Keywords:

Cardiac Tissue Engineering, Connexin, Hydroxyapatite, P19.CL6, Scaffold

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