Interface Function and Cefazolin-Adsorption-Release Characteristics of Hydroxyapatite Granules Modified by Supersonic Treatment Techniques

Abstract:

Commercial hydroxyapatite (HAp) porous granules were designed by the supersonic treatment at 120W and 38 kHz for 15 min in parenteral fluids (amino-acids (PF-A), electrolytes and carbohydrates (PF-EC), the mixtures (PF-M)) to form PF-A/HAp, PF-EC/HAp and PF-M/HAp. The modified HAp kept spherical shape and exhibited adhesive aggregates originated from parenteral fluid components on the surface layer. The BET specific surface areas decreased from 39 m²・g^-1 to 19-24 m²・g^-1. The granules dried at 293K were stirred at 309.5 K in 0.5-2.5 mg・cm^-3 CEZ saline solutions. Adsorption isotherms of CEZ for all the granules almost obeyed the Langmuir type-equation. The amounts of CEZ adsorbed on the modified HAp were larger than those on HAp. Regarding CEZ-release characteristics in a biomimetic environment, the CEZ-adsorbed granules were freeze-dried or dried at 293K and they were stirred at 309.5K and pH 7.40 in simulated body fluid (SBF). The CEZ-release efficiencies for granules freeze-dried into SBF were significantly higher than those for granules dried at 293K. The values of granules freeze-dried were 37-43% for PF-A/HAp, 75-83% for PF-EC/HAp, and 56-64% for PF-M/HAp, which were related to the interface function, such as hydrophilicity of surface and multilayer film thickness of water molecule.

• Periodical: Key Engineering Materials (Volume 696)
• Pages: 265-270
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.696.265
• Online since: May 2016
• Authors: Toshiyuki Akazawa, Masaru Murata, Yasuhito Minamida, Md. Arafat Kabir, Manabu Ito, Michiko Sakamoto, Takehiko Nakajima
• Keywords: Adsorption-Release Characteristics, Cefazolin, Hydroxyapatite, Interface Function, Microstructure, Parenteral Fluids, Supersonic Treatment

References

[1] T. Akazawa, M. Murata, Y. Minamida, A. Kabir, M. Ito, A. Katayama and T. Nakajima, Interface function design and bone-regenerative engineering of biomimetic biomaterials by supersonic treatment using electrolyzed water, Key Eng. Mater., 631 (2014).

DOI: 10.4028/www.scientific.net/kem.631.241

[2] Information on http: /www. md. ucl. ac. be/didac/hosp/ssi. pdf.

[3] Y. Minamida, T. Akazawa, M. Murata, A. Kabir, M. Ito, T. Nakajima, M. Arisue, Preparation of cefazorin/hydroxyapatite granules and the preventive technique for infectious diseases, Abstract of 22nd. Annual meeting of Japanese association of inorganic phosphorus chemistry (2012).

[4] T. Akazawa, M. Murata, Y. Tabata, M. Ito, Microstructure and biocompatibility of hydroxyapatite porous ceramics designed by a partial dissolution-precipitation technique with supersonic treatment for bone regeneration, in: H. Tal (Ed. ), Bone Regeneration, InTech Co., Rijeka, Croatia, 2012, pp.283-300.

DOI: 10.5772/32088

[5] T. Akazawa, M. Murata, T. Nomura, T. Shigyo, K. Sakai, Y. Minamida, Md. A. Kabir, T. Yamagishi, S. Iida, M. Ito, K. Nakamura, S. Miyazaki, Surface design and water vapor-adsorption characteristics of biomimetic composite materials derived from salmon resource, Key Eng. Mater., 529-530 (2013).

DOI: 10.4028/www.scientific.net/kem.529-530.430

[6] T. Akazawa, M. Ito, Controllable and preventive technologies of biofilm and infectious diseases, in: H. Yoshikawa, T. Nakano, A. Matsuoka, Y. Nakajima (Eds), The Development of artificial joints – from the history to future prospects, Inc. NIHON IGAKUKAN, Japan, 2013, pp.315-318.

[7] M. Uchino, K. Sugo, K. Naruse, K. Uchida, N. Hirakawa, M. Toyama, G. Miyajima, K. Urabe, Elution characteristics of vancomycin, gentamicin, and vancomycin/gentamicin combination from calcium phosphate cement, Advances in orthopedic Surgery, 2015 (2015).

DOI: 10.1155/2015/257925

[8] T. Akazawa, T. Nomura, M. Murata, Y. Minamida, M. Ito, T. Nakajima, M. Sakamoto, Functional design and characterization of hydroxyapatite composite granules with cefazolin-release characteristics by a supersonic surface modification method, Abstract of SCEJ 47th. autumn meeting (2015).

DOI: 10.4028/www.scientific.net/kem.696.265