Bio-Electrospraying of Human Umbilical Vein Endothelial Cells with a Customized Multi-Hole Spinneret

Ting Zhang; Yuan Yuan Liu; Hong Chen Yu; Shuai Li; Hai Ping Chen; Qing Xi Hu

doi:10.4028/www.scientific.net/KEM.705.291

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HomeKey Engineering MaterialsKey Engineering Materials Vol. 705Bio-Electrospraying of Human Umbilical Vein...

Bio-Electrospraying of Human Umbilical Vein Endothelial Cells with a Customized Multi-Hole Spinneret

Abstract:

Bio-electrospraying (BES) is becoming an attractive tool for the delivery of cells into scaffolds for tissue engineering applications. In this study, we aimed to electrospray human umbilical vein endothelial cells (HUVECs) and improve the efficiency of BES by designing a new customized multi-hole spinneret. We demonstrated that the multi-hole spinneret could produce continuous and stable jets during BES, and the efficiency was increased by 5–7 times. Morphological observations, trypan blue and sulforhodamine B assays revealed that the HUVECs electrosprayed using the multi-hole spinneret remained viable and proliferated at a rate similar to that of the controls. Thus, the new multi-hole nozzle can considerably improve output for BES without affecting cell morphology, viability, and proliferation.

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Periodical:

Key Engineering Materials (Volume 705)

Pages:

291-296

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.705.291

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Online since:

August 2016

Authors:

Ting Zhang, Yuan Yuan Liu*, Hong Chen Yu, Shuai Li, Hai Ping Chen, Qing Xi Hu

Keywords:

Bio-Electrospraying, Cell Delivery, Human Umbilical Vein Endothelial Cell, Multi-Hole Spinneret, Tissue Engineering

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