Characterization of Structural and Chemical Properties of Silver Containing Hydroxyapatite Synthesized by Solid Phase Reaction

Abstract:

In this study, we investigated the physical and chemical state changes of silver in silver-containing hydroxyapatite (AgHA) composites sintered at various heat treatment temperatures. AgHA composites were synthesized by a solid phase reaction at various temperatures using composite powder prepared by ball-milling commercial highly crystalline hydroxyapatite powder and commercial silver oxide powder. Structural characterization of AgHA was performed by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and X-ray absorption near edge structure (XANES). The XRD measurement showed that peaks of metallic silver or other silver compounds disappeared at 800°C and reappeared at 1000°C. The a-axis of HA obtained by Rietveld analysis expanded drastically at 800°C. Furthermore, the SEM images showed that silver particles disappeared at 800°C and reappeared at 1000°C, as indicated by the XRD patterns. The XANES spectrum of Ag-L₃ edge of AgHA sintered at 800°C was different compared to those of other references. These results suggested that calcium ions were substituted by silver ions in AgHA sintered at 800°C, according to the a-axis expansion of HA lattice caused by the substitution of hydroxyl ions by carbonate ions through the heat treatment, based on the FT-IR measurement. In addition, antibacterial tests showed that AgHA containing various chemical states of silver had good antibacterial activities in proportion to released amounts of silver.

• Periodical: Key Engineering Materials (Volume 720)
• Pages: 3-6
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.720.3
• Online since: November 2016
• Authors: Takayuki Murakami*, Iwao Noda, Junji Ikeda, Atsushi Nakahira
• Keywords: Antibacterial, Carbonate Ions, FT-IR, Silver, XRD

References

[1] G. Gosheger, et al., Biomaterials, 25, (2004) 5547-5556.

[2] S. Samani, et al., J. Biomed. Mater. Res. Part A, 101A, (2013) 222-230.

[3] NM. Zain, et al., Carbohyr. Polym., 112, (2014) 195-202.

[4] I. Noda, et al., J. Biomed. Mater. Res. Part B Appl. Biomater., 89B, (2009) 456-465.

[5] L. Badrour, et al., Ann. Chim. Sci. des Mater., 23, (1998) 61-64.

[6] N. Rameshbabu, et al., J. Biomed. Mater. Res. Part A, 80A, (2007) 581-591.

[7] Y. Ando, et al., Mater. Sci. Eng., C, 30, (2010) 175-180.

[8] RZ. LeGeros, et al., An Introduction to Bioceramics, LL. Hench et al. ed., Singapore, World Science, (1993) 139-180.

[9] S. Eto, et al., J Orthop Res., 33, (2015) 1391-1397.