Electrodeposition of Chitosan Composite Film and its Application for Protein Conjugation

Ting Chen; Cui Li Xiang; Fen Xu; Li Xian Sun

doi:10.4028/www.scientific.net/KEM.748.103

Paper Titles

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Biocompatible Tough Hydrogels via Micellar Copolymerization of NIPAM and Stearyl Acrylate: Synthesis and Characterization
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Electrodeposition of Chitosan Composite Film and its Application for Protein Conjugation
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 748Electrodeposition of Chitosan Composite Film and...

Electrodeposition of Chitosan Composite Film and its Application for Protein Conjugation

Abstract:

Developing sensitive, reliable and simple methods for detection of protein biomarkers in complex biological matrixes is a major challenge in recent years. In most cases, immunoassays are performed in muti-well-plates in well-equipped labs, which is time consuming and expensive. There is great interest in transitioning immunoanalysis from the lab so the analytical capabilities of immunoanalysis can be employed to diagnose disease at the point-of-care and monitor the environment in the field. In immunoassay applications, antibodies are immobilized onto biointerface to capture specific biomarkers. To improve the sensitivity and reduce the size of immunosensor, immobilizing antibody at high density is essential. Here, we developed an easier method to conjugate the antibodies within the chitosan composite film and remove the non-specific binding with electrical field.

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Advanced Materials and Engineering Materials VI

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Periodical:

Key Engineering Materials (Volume 748)

Pages:

103-107

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.748.103

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Online since:

August 2017

Authors:

Ting Chen, Cui Li Xiang*, Fen Xu, Li Xian Sun

Keywords:

Chitosan, Composite Material, Electrodeposition

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References

[1] H.H. Ma, J.Z. Sun, Y. Zhang, S.H. Xia, Disposable amperometric immunosensor for simple and sensitive determination of aflatoxin B-1 in wheat, Biochem. Eng. J. 115(2016)38-46.

DOI: 10.1016/j.bej.2016.08.003

Google Scholar

[2] C. Xiang, R. Li, B. Adhikari, Z. She, Y. Li, H.B. Kraatz, Sensitive electrochemical detection of Salmonella with chitosan–gold nanoparticles composite film, Talanta 140(2015)122-127.

DOI: 10.1016/j.talanta.2015.03.033

Google Scholar

[3] A.M.A.A.A. AL-Mokaram, R. Yahya, M.M. Abdi, H.N.M.E. Mahmud, One-step electrochemical deposition of Polypyrrole-Chitosan-Iron oxide nanocomposite films for non-enzymatic glucose biosensor, Mater. Lett. 183(2016)90-93.

DOI: 10.1016/j.matlet.2016.07.049

Google Scholar

[4] L. Pereira, A. Sousa, H. Coelho, A. M. Amado, P. J. A. Ribeiro-Claro, Use of FTIR, FT-Raman and (13)GNMR spectroscopy for identification of some seaweed phycocolloids, Biomolecular Eng. 20(2003)223-228.

DOI: 10.1016/s1389-0344(03)00058-3

Google Scholar