Evaluation of Protein Adsorption Capacity of TiO₂-Supported Spherical Porous Hydroxyapatite

Abstract:

In this study, a novel titania-supported spherical porous hydroxyapatite (sHAp + TiO₂) was prepared by compounding minute, uniquely shaped spherical porous hydroxyapatites (sHAp) with TiO₂. Adsorption of the representative protein bovine serum albumin (BSA) was evaluated and simulated body fluid immersion experiments were carried out. Minimal adsorption of BSA to TiO₂ was observed, and 33% and 25% adsorption to sHAp + TiO₂ and sHAp were observed, respectively. The reduction in BSA concentration observed in the sHAp and sHAp + TiO₂ solutions is presumably due to adsorption to HAp. It is highly likely that adsorption of the acidic protein BSA occurred at the sHAp Ca²⁺ site. In the simulated body fluid immersion experiments, there was greater expression of hydroxyapatite (HAp) on the surface of sHAp than there was on the surface of sHAp + TiO₂. In the case of TiO₂ alone, no HAp was produced, even after immersion for 3 days. These results suggest a relationship between BSA adsorption and the osteoconductivity of materials.

• Periodical: Key Engineering Materials (Volume 758)
• Pages: 90-96
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.758.90
• Online since: November 2017
• Authors: Max Katayama*, Kengo Tamazawa, Ayu Kodaira, Toru Nonami
• Keywords: BSA, Hydroxyapatite, Simulated Body Fluid (SBF), TiO₂

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