Synthesis of Europium(III) Complex-Based Hydroxyapatite Nanocrystals for Biolabeling Applications

Takuya Kataoka; Shigeaki Abe; Motohiro Tagaya

doi:10.4028/www.scientific.net/KEM.782.41

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Synthesis of Europium(III) Complex-Based Hydroxyapatite Nanocrystals for Biolabeling Applications
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 782Synthesis of Europium(III) Complex-Based...

Synthesis of Europium(III) Complex-Based Hydroxyapatite Nanocrystals for Biolabeling Applications

Abstract:

We synthesized the inorganic/organic hybrid nanocrystal (EHA) by hydroxyapatite (HA) nanocrystal growth under the existence of tris(2,2,6,6-tetramethyl-3,5-heptanedionato)europium(III) (EuTH) complex. Then, folic acid derivative (FA-NHS: folate N-hydroxysuccinimidyl ester) as the targeting ligand for the HeLa cancer cells was immobilized on the EHA by the mediation of 3-aminopropyltriethoxysilane (APTES) and methyltriethoxysilane (MTES). When the FA-NHS molecular occupancy ratio on the EHA surface is around 3 to 5 %, the strong luminescence from the f-f transition of the Eu³⁺ ion and luminescence associated with energy transfer between the EuTH-FA monomer near 518 nm were observed. Moreover, the dispersibility in phosphate buffer saline was confirmed with immobilizing the positively-charged FA-NHS. The affinity and non-cytotoxicity between the nanocrystals and HeLa cancer cells were confirmed for 3 days. The red luminescence from the cells could be observed by fluorescence microscopy and the luminescence spectra.

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Key Engineering Materials (Volume 782)

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41-46

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.782.41

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Online since:

October 2018

Authors:

Takuya Kataoka, Shigeaki Abe, Motohiro Tagaya*

Keywords:

Bio-Labeling, Europium (III) Complex, Exciplex Emission, HeLa Cancer Cells, Hydroxyapatite, Organic-Inorganic Nanohybrid

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