Development of Clove Oil In Situ Gels for Buccal Mucoadhesive

Abstract:

Clove oil is the volatile essential oil obtained from Syzygium aromaticum. Eugenol, which is the main component, has antimicrobial, antifungal activity and antioxidant. From antiseptic activity, clove oil can be used in treatment of oral cavity infection. The aims of this research are to formulate and evaluate clove oil mucoadhesive gel for oral applications. Gel bases were prepared from Poloxamer 407 (P407). The effect of clove oil, P407 and xanthan gum on formulations were studied. Clove oil preparations were evaluated for their physicochemical properties (physical appearance, pH, viscosity, gelling capacity and gel dissolving time at 37 °C in artificial saliva). Mucoadhesive property was studied using porcine buccal mucosa. The amount of eugenol in clove oil was analyzed by gas chromatography (GC). Stability study of clove oil gel was performed under stress and room temperature conditions (1 month). The results showed that preparations containing 0% and 1% clove oil were in situ gel while those containing 3% and 5% clove oil were gel. All formulations showed good and clear physical appearance with the pH value of 5.2. The gelling capacity and gel dissolving time of more than 15 minutes in artificial saliva at 37°C was found in all preparations. They also showed mucoadhesive property. The GC results showed that the amount of eugenol in clove oil were 99.52 ± 0.51 %V/V. The preparations containing 1% clove oil showed good physical and chemical stability after storage at room temperature for 1 month. There was no significant difference in the amount of eugenol after storage (p>0.05). In conclusion, the preparations containing 20% P407 and 1% clove oil are the most appropriate formulation.

• Periodical: Key Engineering Materials (Volume 859)
• Pages: 57-61
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.859.57
• Online since: August 2020
• Authors: Suwannee Panomsuk*, Kunyakorn Keawsri, Chanapa Limsatjapanit, Nutcha Asaneesantiwong, Parichat Chomto, Malai Satiraphan
• Keywords: Buccal Mucoadhesive, Clove Oil, Eugenol, In Situ Gels, Stability

References

[1] D.F. Cortés-Rojas, C.R. Fernandes de Souza and W.P. Oliveira, Clove (Syzygium aromaticum): a precious spice, Asian Pac. J. Trop. Biomed. 4(2) (2014) 90-96.

DOI: 10.1016/s2221-1691(14)60215-x

[2] K. Chaieb, H. Hafedh Hajlaoui, T. Zmantar, A.B. Kahla-Nakbi, M. Rouabhia, K. Mahdouani and A. Bakhrouf, The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): a short review, Phytother. Res. 21 (2007) 501–506.

DOI: 10.1002/ptr.2124

[3] I Gülçin, M Elmastaş, H.Y. Aboul-Enein, Antioxidant activity of clove oil, A powerful antioxidant source. Arab. J. Chem. 5 (2012) 489–499.

DOI: 10.1016/j.arabjc.2010.09.016

[4] T. Nagai and R. Konishi, Buccal gingival drug delivery system, J. Control. Release. 6 (1987) 353-360.

DOI: 10.1016/0168-3659(87)90088-5

[5] A.J.H. Janet, W.W. Philip and W.W. Philip, Drug delivery via the buccal mucosa, Pharm. Sci. Tech. Today. 7(1) (1998), pp.309-316.

[6] J. Byeongmoon, W.K. Sung and H.B. You, Thermosensitive sol–gel reversible hydrogels, Adv. Drug Del. Rev. 54 (2002) 37-51.

[7] N.M. Hrisah, P. Prabhu, R.N. Charyulu, A. Gulzar and E.V.S. Subrahmanyam, Formulation and evaluation of in situ gels containing clotrimazole for oral candidiasis, Indian J. Pharm. Sci. 71(4) (2009) 421-427.

DOI: 10.4103/0250-474x.57291

[8] R.I. Burks, Povidone-iodine solution in wound treatment, Phys. Ther. 78(2) (1998) 212-218.

DOI: 10.1093/ptj/78.2.212

[9] K. Nagahama, Y. Imai, T. Nakayama, J. Ohmura, T. Ouchi and Y. Ohya, Thermo-sensitive sol-gel transition of poly(depsipeptide-co-lactide)-g-PEG copolymers in aqueous solution, Polymers. 50 (2009) 3547-55.

DOI: 10.1016/j.polymer.2009.05.045

[10] K. Małolepsza-Jarmołowska, The effect of poloxamer 407 on the properties of hydrophilic gels containing lactic acid complexed with chitosan, Progress in chemistry and application of chitin and its derivatives (PCACD). 15 (2010) 143-148.

DOI: 10.15259/pcacd.25.011

[11] T.L. Lucas, H.B. Rafik and H.S. Robers, A stability program for the distribution of drug products, Pharm. Technol. July (2004) 68-72.

[12] S. Panomsuk, S. Indis, P. Panyasaroj, S. Chootrakulwattana, P. Opanasopit and T. Rojanarata, Oral bases containing Centella asiatica extract: formulations and evaluations, Adv. Mat. Res. 506 (2012) 501-504.

DOI: 10.4028/www.scientific.net/amr.506.501

[13] B.Y.K. Sruthi, B.M. Gurupadayya, K.V. Sairam, T.N. Kumar, Development and validation of GC method for the estimation of eugenol in clove extract, Int. J. Pharm. Sci, 6(2) (2014) 473-476.