Evaluation of Potential of Uncoated Mesoporous Silica Particles for Drug Delivery Applications

Abstract:

The drug loading capability and inherent cytotoxicity of mesoporous silica particles are two prime considerations for targeted drug delivery applications. In current study, uncoated mesoporous silica (UMS) carrier particles were synthesized by sol-gel emulsion approach. The morphology and structure of UMS was thoroughly characterized using atomic force microscope (AFM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Brunauer–Emmett–Teller (BET). The scanning electron microscopy (SEM) and dynamic light scattering (DLS) measurements reveal that mono dispersed silica particles have an average size of 250 nm with narrow size distribution. The pore size was measured as 47nm. Concentration dependent biocompatibility of UMS was evaluated using MTT assay with Hep-2c cancer cell line and cell viability of ~65% at concentrations of 7.5 nM was observed. Finally, the drug loading capability of UMS carrier was studied using ibuprofen as a model drug.

• Periodical: Key Engineering Materials (Volume 875)
• Pages: 366-372
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.875.366
• Online since: February 2021
• Authors: Tayyab Ali Khan*, Syed Mujtaba Ul Hassan, Hassan Waqas, Jamil Ahmad, Ahmat Khurshid, Mazhar Mehmood, Muhammad bin Ahmed, Sumaira Naz
• Keywords: Drug Delivery, Mesoporous, Sol-Gel, Theranostic, Uncoated Mesoporous Silica (UMS)

References

[1] R. Devi, P.R. Devi, K.G. Dhanalakshmi, Applications of mesoporous silica nanomaterial : an over view, Int. J. Adv. Life Sci., 4(2012) 1–9.

[2] Y. Zhang, B.Y.W. Hsu, C. Ren, X. Li, J. Wang, Silica-based nanocapsules: Synthesis, structure control and biomedical applications, Chem. Soc. Rev. 44 (2015) 315–335.

DOI: 10.1039/c4cs00199k

[3] N. Hao, L. Li, F. Tang, Shape-mediated biological effects of mesoporous silica nanoparticles, J. Biomed.Nanotechnol.10 (2014) 2508–2538.

DOI: 10.1166/jbn.2014.1940

[4] Z. Teng, Y. Han, J. Li, F. Yan, W. Yang, Preparation of hollow mesoporous silica spheres by a sol-gel/emulsion approach, Microporous Mesoporous Mater., 12(2010) 67–72.

DOI: 10.1016/j.micromeso.2009.06.028

[5] H.M. Abdelaal, Fabrication of hollow silica microspheres utilizing a hydrothermal approach, Chinese Chem. Lett. 25(2014) 627–629.

DOI: 10.1016/j.cclet.2014.01.043

[6] Y. Yang, C. Yu, Advances in silica based nanoparticles for targeted cancer therapy, Nanomed. Nanotech. Biol. Med.12(2015) 317–332.

[7] W.H. De Jong, P.J.A. Borm, Drug delivery and nanoparticles : Applications and hazards, Int. J. Nanomedicine 3 (2008) 133–149.

[8] A.C. Anselmo et al., Elasticity of Nanoparticles Influences Their Blood Circulation, Phagocytosis , Endocytosis and Targeting, ACS Nano, (2015).

DOI: 10.1021/acsnano.5b00147

[9] I. Rukshin, J. Mohrenweiser, P. Yue, S. Afkhami, Modeling Superparamagnetic Particles in Blood Flow, Fluids, 2. (2017) 1–12.

DOI: 10.3390/fluids2020029

[10] L.R. Shobin, D. Sastikumar, S. Manivannan, Sensors and Actuators A : Physical Glycerol mediated synthesis of silver nanowires for room temperature ammonia vapor sensing, Sensors Actuators A. Phys. 214 (2014) 74–80.

DOI: 10.1016/j.sna.2014.04.017

[11] M. Chen, A. Von Mikecz, Formation of nucleoplasmic protein aggregates impairs nuclear function in response to SiO2 nanoparticles, Exp. Cell Res. 305(2005) 51–62.

DOI: 10.1016/j.yexcr.2004.12.021

[12] Y.S. Lin, C.L. Haynes, Impact of mesoporous silica nanoparticles and pore integrity on hemolytic activity, J. Am. Chem. Soc. 132 (2010) 4834–4842.

DOI: 10.1021/ja910846q

[13] H.F. Krug, R. Wottrich, S. Diabate, Biological effects of ultrafine model particles in human macrophages and epithelial cells in mono- and co-culture, Int. J. Hyg. Environ. Health 207 (2004) 353–361.

DOI: 10.1078/1438-4639-00300

[14] T.J. Brunner, P. Wick, A. Bruinink, In Vitro Cytotoxicity of Oxide Nanoparticles : Comparison to Asbestos, Silica, and the Effect of Particle Solubility, Environ. Sci. Technol. 40 (2006) 4374–4381.

DOI: 10.1021/es052069i

[15] X. Huang et al., The shape effect of mesoporous silica nanoparticles on biodistribution, clearance, and biocompatibility in vivo, ACS Nano 5 (2011) 5390–5399.

DOI: 10.1021/nn200365a

[16] C. Line, In Vitro Cytotoxicitiy of Silica Nanoparticles at High Concentrations Strongly Depends on the Metabolic Activity Type of the Cell Line, Environ. Sci. Technol. 41 (2007) 2064–(2068).

DOI: 10.1021/es062347t.s001

[17] M.A. Rida, F. Harb, Synthesis and Characterization of Amorphous Silica Nanoparitcles from Aqueous Silicates using Cationic Surfactants, J. Met. Mater. Miner. 24 (2014) 37–42.

[18] B.U. Yoo, M.H. Han, H.H. Nersisyan, J.H. Yoon, K.J. Lee, J.H. Lee, Self-templated synthesis of hollow silica microspheres using Na2SiO3 precursor, Microporous Mesoporous Mater. 190 (2014) 139–145.

DOI: 10.1016/j.micromeso.2014.02.005

[19] A. Petushkov, N. Ndiege, A.K. Salem, S.C. Larsen, Toxicity of silica nanomaterials: Zeolites, mesoporous silica, and amorphous silica nanoparticles, Adv. Molec. Toxic. 4(2010) 223-266.

DOI: 10.1016/s1872-0854(10)04007-5

[20] K.Walczak-zeidler, I. Nowak, UV-Vis and IR techniques for drug identification in porous carriers, Chemik 65 (2011) 643–648.