Behavior of Osteoclast Cells Response on Dicalcium Phosphate Dihydrate Layer-Coated β-Tricalcium Phosphate Granular

Abstract:

The aim of this study is to investigate the behavior of osteoclast cells response on dicalcium phosphate dihydrate (DCPD) layer-coated β-TCP granules. β-TCP granules with 300-600 μm were exposed to acidic calcium phosphate solution for 30 mins in order to get 10 mol% DCPD layer-coated β-TCP granular. DCPD free-coated β-TCP granular had used as control specimen. Both specimens were implant in 9 mm of rat calvarial bone defect for 4 weeks. After 4 weeks, the block section of rat calvarial containing specimen were removed for Tatrate-Resistance Acid Phosphatase (TRAP) analysis. Results of TRAP staining reveal that the number of osteoclast cells attached on 10 mol% layer-coated β-TCP granular is higher than DCPD free-coated β-TCP granular. Since remodeling of new bone formation involved simultaneous osteoclast and osteoblast cells response, therefore, the results obtained in this study indicated that the presence of DCPD layer-coated on β-TCP granular helps to improve osteoclast cells response that contribute in stimulating new bone formation.

• Periodical: Materials Science Forum (Volume 1010)
• Pages: 549-554
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.1010.549
• Online since: September 2020
• Authors: Khairul Anuar Shariff*, Mohamad Hafizi Abu Bakar, Arief Cahyanto
• Keywords: Bioceramic, Bone Remodeling, Calcium Phosphate, Ceramic Coating, Osteoclast Cell

References

[1] G. Robert, C.R. Gwendolen, In-vitro models of bone remodelling and associated disorders, Frontiers in Bioengineering and Biotechnology 6 (2018) 1-22.

[2] K.J. Oh, Y.B. Ko, S. Jaiswal, Comparison of osteoconductivity and absorbability of beta-tricalcium phosphate and hydroxyapatite in clinical scenario of opening wedge high tibial osteotomy, Journal of Material Science: Materials in Medicine 27 (2016) 1-7.

DOI: 10.1007/s10856-016-5795-1

[3] I. Kunio, M. Youji, T. Akira, H. Koichiro, T. Kanji, O. Go, Physical and histological comparison of hydroxyapatite and β-Tricalcium Phosphate Bone Substitutes, Materials 11(2018) 1-12.

[4] S. Khairul Anuar, T. Kanji, I. Kunio, Fabrication of dicalcium phosphate dihydrate coated β-TCP granules and evaluation of their osteoconductivity using experimental rats, Materials Science and Engineering C 75 (2017) 1411-1419.

DOI: 10.1016/j.msec.2017.03.004

[5] M. Zaki, K.A. Shariff, M. Hafizi, M. Nurul Azmi, Effect of Different Granular Size on the Properties of Porous β-Tricalcium Phosphate Foam Granular Cements, Key Engineering Materials 829 (2020) 23-27.

DOI: 10.4028/www.scientific.net/kem.829.23

[6] R.A. Timothy, Extracellular pH regulates bone cell function, The Journal of Nutrition 138 (2008) 415S-418S.

DOI: 10.1093/jn/138.2.415s

[7] S. Ghanaati, M. Barbeck, C. Orth, I. Willershausen, B.W. Thim, C. Hoffman, A. Rasic, R.A. Sader, R.E. Unger, F. Peters, C.J. Kirkpatrick, Influence of β-tricalcium phosphate granule size and morphology on tissue reaction in-vivo, Acta Biomaterilia 6 (2010) 4477-4487.

DOI: 10.1016/j.actbio.2010.07.006

[8] N. Rucci, Molecular biology of bone remodeling, Clinical Cases in Mineral and Bone Metabolism, 5(1) (2008) 49-56.

[9] D. J. Hadjidakis, I. I. Androulakis, Bone remodeling, Annals of New York Academy of Science, 1092 (2006) 385-396.

DOI: 10.1196/annals.1365.035