Rapid and Visual Detection of Vitamin C Based on Sodium Citrate-Stabilized Silver Nanoparticles

Abstract:

Ensuring the vitamin C requirements are met through dietary intake or supplements is essential for health. In this study, a simple and rapid visual detection method for semi-quantitative analysis of vitamin C was proposed based on sodium citrate-stabilized silver nanoparticles (SC-AgNPs). As a validation of SC-AgNPs as a colorimetric sensor for vitamin C, color changes were observed gradually, shifting from transparent yellow to deep brown after the addition of vitamin C at various concentrations. That is also supported by an increase in absorption intensity at the peak wavelength of 417 nm, which was analyzed using a UV-Vis spectrophotometer. Based on the spectral approach method, the linear relationship between absorbance values and vitamin C is in the range of 0-4.2 mM, with a coefficient correlation of 0.99 and sensitivity of 0.95/mM. Furthermore, the feasibility of the SC-AgNPs for vitamin C detection in healthy beverages and tablet pharmaceuticals has been investigated.

• Periodical: Materials Science Forum (Volume 1118)
• Pages: 21-28
• DOI: https://doi.org/10.4028/p-a2DGQn
• Online since: March 2024
• Authors: Indah Nursyamsi Handayani, Vivi Fauzia*, Adhi Harmoko Saputro, Nursama Heru Apriantoro
• Keywords: Colorimetric, Silver Nanoparticles, Sodium Citrate, Vitamin C

References

[1] A.G. Godswill, I.V. Somtochukwu, A.O. Ikechukwu, and E.C. Kate, "Health Benefits of Micronutrients (Vitamins and Minerals) and their Associated Deficiency Diseases: A Systematic Review," Int. J. Food Sci., vol. 3, no. 1, p.1–32, 2020.

DOI: 10.47604/ijf.1024

[2] A. Akbari, G. Jelodar, S. Nazifi, and J. Sajedianfard, "An Overview of the Characteristics and Function of Vitamin C in Various Tissues: Relying on its Antioxidant Function," Zahedan J. Res. Med. Sci., vol. In Press, no. In Press, 2016.

DOI: 10.17795/zjrms-4037

[3] M. Doseděl et al., "Vitamin c—sources, physiological role, kinetics, deficiency, use, toxicity, and determination," Nutrients, vol. 13, no. 2, p.1–36, 2021.

DOI: 10.3390/nu13020615

[4] F. Susa and R. Pisano, "Advances in Ascorbic Acid ( Vitamin C ) Manufacturing : Green Extraction Techniques from Natural Sources," no. Vitam. C, 2023.

DOI: 10.3390/pr11113167

[5] S. J. Devaki and R. L. Raveendran, "Vitamin C: Sources, Functions, Sensing and Analysis," Vitam. C, no. August, 2017.

DOI: 10.5772/intechopen.70162

[6] S. Sumra, N. Humera, A. Asma, S. Muhammad, and R. Shah, "Sensitive and highly selective colorimetric biosensing of vitamin ‑ C and vitamin ‑ B1 by flavoring agent ‑ based silver nanoparticles," JBIC J. Biol. Inorg. Chem., no. 0123456789, 2022.

DOI: 10.1007/s00775-022-01944-5

[7] L. Huang, S. Tian, W. Zhao, K. Liu, and J. Guo, "Electrochemical vitamin sensors: A critical review," Talanta, vol. 222, no. August 2020, p.121645, 2021.

DOI: 10.1016/j.talanta.2020.121645

[8] E. Mauriz, "Colorimetric Sensors 2 . Distance – Dependent Plasmonic Naked-Eye Colorimetric 3 . Colorimetric Sensors Based on Etching and Growth of Noble Metal Nanoparticles," p.2017–2020, 2014.

DOI: 10.3390/s20216214

[9] A. Kumari, V. Vyas, and S. Kumar, "Advances in electrochemical and optical sensing techniques for vitamins detection: a review," ISSS J. Micro Smart Syst., vol. 11, no. 1, p.329–341, 2022.

DOI: 10.1007/s41683-021-00084-3

[10] A. Othman, L. Norton, A. S. Finny, and S. Andreescu, "Easy-to-use and inexpensive sensors for assessing the quality and traceability of cosmetic antioxidants," Talanta, vol. 208, p.1–22, 2020.

DOI: 10.1016/j.talanta.2019.120473

[11] G. Mushrooms et al., "We are IntechOpen , the world ' s leading publisher of Open Access books Built by scientists , for scientists TOP 1 %," Intech, vol. i, no. 2, p.13, 2022, [Online]. Available: https://doi.org/10.1016/j.jfutfo.2022.09.004%0A https://www.redalyc.org/ pdf/685/68514101.pdf%0A https://doi.org/10.1007/s13668-023-00468-x%0Ahttps://suite.io/ philip-mcintosh/1fjv2v4.

[12] M. Sabela, S. Balme, M. Bechelany, J. M. Janot, and K. Bisetty, "A Review of Gold and Silver Nanoparticle-Based Colorimetric Sensing Assays," Adv. Eng. Mater., vol. 19, no. 12, 2017.

DOI: 10.1002/adem.201700270

[13] G. Alberti, C. Zanoni, L. R. Magnaghi, and R. Biesuz, "Gold and silver nanoparticle-based colorimetric sensors: New trends and applications," Chemosensors, vol. 9, no. 11, 2021.

DOI: 10.3390/chemosensors9110305

[14] R. Roto, H. P. Rasydta, A. Suratman, and N. H. Aprilita, "Effect of reducing agents on physical and chemical properties of silver nanoparticles," Indones. J. Chem., vol. 18, no. 4, p.614–620, 2018.

DOI: 10.22146/ijc.26907

[15] D. S. Menamo, D. W. Ayele, and M. T. Ali, "Green synthesis, characterization and antibacterial activity of copper nanoparticles using L-ascorbic acid as a reducing agent," Ethiop. J. Sci. Technol., vol. 10, no. 3, p.209, 2017.

DOI: 10.4314/ejst.v10i3.5

[16] P. S. Yerragopu, S. Hiregoudar, U. Nidoni, K. T. Ramappa, A. G. Sreenivas, and S. R. Doddagoudar, "Chemical Synthesis of Silver Nanoparticles Using Tri-sodium Citrate, Stability Study and Their Characterization," Int. Res. J. Pure Appl. Chem., no. March, p.37–50, 2020.

DOI: 10.9734/irjpac/2020/v21i330159

[17] D. Acharya, B. Mohanta, S. Deb, and A. K. Sen, "Theoretical prediction of absorbance spectra considering the particle size distribution using Mie theory and their comparison with the experimental UV–Vis spectra of synthesized nanoparticles," Spectrosc. Lett., vol. 51, no. 3, p.139–143, 2018.

DOI: 10.1080/00387010.2018.1442351