The Effects of Different Hydroxyapatite/TiO2 Composite Coatings on Protein Expression of Osteoblast

Jian Ye Han; Zhen Tao Yu; Lian Zhou

doi:10.4028/www.scientific.net/MSF.610-613.1104

Paper Titles

Quantification of BSA in Tissue Engineered Medical Products by ELISA — Problems and Solutions
p.1076

Surface Characteristics and Hemocompatibility of Modified NiTi Alloy Intravascular Stents
p.1082

Study on Biomineralization of Poly (Vinyl Alcohol) Film via a Biomimetic Process
p.1090

Preparation and Performance of Polypyrrole Film with Micro-Antennal Morphology for Medical Biology
p.1096

The Effects of Different Hydroxyapatite/TiO₂ Composite Coatings on Protein Expression of Osteoblast
p.1104

Preparation, Microstructure and Properties of Biodegradable Aliphatic Polyesters by Using Nontoxic Magnesium Catalysts
p.1109

Neovascularization of Porous Silk Fibroin Films(PSFFs) as Dermis Substitutes Implanted in Rats
p.1113

High-Cycle Fatigue Mechanisms of a NiTi Shape Memory Alloy under Different Mean Strains
p.1120

Influence of the Flux Ratio N₂/Ar on the Formation of Mo-N Modification Layers on Ti₆Al₄V by Plasma Reactive Sputtering
p.1128

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The Effects of Different Hydroxyapatite/TiO₂ Composite Coatings on Protein Expression of Osteoblast

Abstract:

Hydroxyapatite/TiO2 composite material was coated onto Ti25Nb3Mo2Sn3Zr (TLM) alloy substrate. To study the effects of hydroxyapatite/TiO2 composite coatings on bone-related protein expression, the osteoblast were cultured with composite coatings for different times. The phase transformation and compound formation of the HA/TiO2 coatings were investigated using XRD (X-ray diffraction). The mRNA expression of Type I collagen, alkaline phosphatase (ALP) and osteocalcin were studied by RT-PCR (reverse transcriptional polymerase chain reaction). The titania delayed the crystallization of HA. The mRNA expressions of Type I collagen are decreased as the increasing of TiO2 percentage. The mRNA expressions of osteocalcin are approached. The ALP expression on H4 coating (HA/TiO2 mol ration is 5) after the osteoblast cultured with composite coating for 6 days is the highest. The increasing of TiO2 amount decreases the bioactivity of the composite coatings.