Study of Highly Selective and Sensitive Microarray Structure Based on Hydrophilic/Hydrophobic SAMs (Self-Assembled Monolayers)

Abstract:

A microarray-based immunoassay requires only small volume of the sample in each array spot, which leads to faster completion of the immunoreactions than with an ELISA (enzyme-linked immunosorbent assay) [. However, this method still has some technical limitations such as poor signal sensitivity, poor detection limit, large sample consumption, high evaporation rate, coffee stain effect, low signal/noise ratio (SNR) and poor quality images. Recently, SAM (Self-Assembled Monolayer) method was extensively researched for control the surface wettability [. Especially, hydrophobic SAM functionalizations which has low surface energy are possible to prevent sample evaporation [ and to achieve anti-biofouling on them. Therefore, an introduction of hydrophobic SAM would enhance the detection sensitivity on bio array chip [4]. In this work, we fabricated a highly selective and sensitive pre-patterned microarray structure by using MEMS (Micro-Electro-Mechanical-System) process and hydrophilic/hydrophobic SAMs with various precursors.

• Periodical: Solid State Phenomena (Volume 195)
• Pages: 82-85
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.195.82
• Online since: December 2012
• Authors: Jung Hwan Lee, Si Hyeong Cho, Hyun Woo Lim, Sung Woo Kim, Ahmed A. Busnaina, Hea Yeon Lee, Jin Goo Park
• Keywords: Fluorescence Analysis, Microarray, Self-Assembled Monolayer (SAM)

References

[1] P. Angenendt, Drug Discov. Today 10 (2005), pp.503-511.

[2] T. Kawai, M. Suzuki, and T. Kondo, Langmuir 22 (2006), pp.9957-9961.

[3] J. M. Moran-Mirabal, C. P. Tan, R. N. Orth, E. O. Williams, H. G. Craighead and D. M. Lin, Anal. Chem., 79 (2007), pp.1109-1114.

DOI: 10.1021/ac061898z

[4] S. Yeo, T. Kwon, C. Choi, H. Park, J. W. Hyun and D. Jung, Curr. Appl. Phys., 6 (2006), pp.267-270.