Study of Highly Selective and Sensitive Microarray Structure Based on Hydrophilic/Hydrophobic SAMs (Self-Assembled Monolayers)

Abstract:

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A microarray-based immunoassay requires only small volume of the sample in each array spot, which leads to faster completion of the immunoreactions than with an ELISA (enzyme-linked immunosorbent assay) [. However, this method still has some technical limitations such as poor signal sensitivity, poor detection limit, large sample consumption, high evaporation rate, coffee stain effect, low signal/noise ratio (SNR) and poor quality images. Recently, SAM (Self-Assembled Monolayer) method was extensively researched for control the surface wettability [. Especially, hydrophobic SAM functionalizations which has low surface energy are possible to prevent sample evaporation [ and to achieve anti-biofouling on them. Therefore, an introduction of hydrophobic SAM would enhance the detection sensitivity on bio array chip [4]. In this work, we fabricated a highly selective and sensitive pre-patterned microarray structure by using MEMS (Micro-Electro-Mechanical-System) process and hydrophilic/hydrophobic SAMs with various precursors.

Info:

Periodical:

Solid State Phenomena (Volume 195)

Edited by:

Paul Mertens, Marc Meuris and Marc Heyns

Pages:

82-85

Citation:

J. H. Lee et al., "Study of Highly Selective and Sensitive Microarray Structure Based on Hydrophilic/Hydrophobic SAMs (Self-Assembled Monolayers)", Solid State Phenomena, Vol. 195, pp. 82-85, 2013

Online since:

December 2012

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$38.00

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