Use of Specific Metal Binding of Self-Assembling S-Layer Proteins for Metal Bioremediation and Recycling

Abstract:

Most bacteria and all archaea possess as outermost cell envelope so called surface-layers (S-layers). These layers were formed by self-assembling proteins having a number of habitat depending interesting intrinsic properties. As example, S-layers from bacterial isolates recovered from heavy metal contaminated environments have outstanding metal binding properties and are highly stable. Thus they selectively bind several metals with different affinity. For using S-layer proteins for metal bioremediation and recycling three aspects of the metal-interactions with S-layer proteins must be taken into account. First, S-layers possess different functionalities, e.g. carboxyl, phosphoryl groups, binding toxic metals and metalloids, like U(VI) and As(V), nonspecifically depending on pH. Second, precious metals like Au and Pd are likewise nonspecifically bound to functional groups, but presumably covalently, making the binding irreversible. Third, intrinsic specifically bound metals, e.g. Ca²⁺, are needed for native protein folding, self-assembly, and the formation of highly-ordered lattices. Their binding sites also allow selective binding of chemical-equal elements including the trivalent rare earth elements, possessing comparable ionic radii. Thus this study combines older and recently generated results regarding the metal dependent binding behavior of the S-layer proteins. It enables the development of biohybrid materials for the separation, removal or recovery of strategic relevant metals from natural occurring or industrial waste waters using pH-value as regulating parameter for selective metal binding and also conceivably release.

• Periodical: Solid State Phenomena (Volume 262)
• Pages: 389-393
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.262.389
• Online since: August 2017
• Authors: Manja Vogel, Sabine Matys, Falk Lehmann, Björn Drobot, Tobias Günther, Katrin Pollmann*, Johannes Raff
• Keywords: Bioremediation, Metal, Recycling, Self-Assembly, S-Layer

References

[1] U. B. Sleytr, P. Messner, d. Pum, M. Sara, Crystalline bacterial cell surface layers (S layers): From supramolecular cell structure to biomimetics and nanotechnology, Angew. Chem-Int Edit 38 (1999) 1035–1054.

DOI: 10.1002/(sici)1521-3773(19990419)38:8<1034::aid-anie1034>3.0.co;2-#

[2] K. Pollmann, J. Raff, M. Merroun, K. Fahmy, S. Selenska-Pobell, Metal binding by bacteria from uranium mining waste piles and its technological applications, Biotechnol. Adv. 24 (2006) 58–68.

DOI: 10.1016/j.biotechadv.2005.06.002

[3] M. Suhr, N. Unger, K. E. Viacava, T. J. Günther, J. Raff, K. Pollmann, Investigation of metal sorption behavior of Slp1 from Lysinibacillus sphaericus JG-B53: a combined study using QCM-D, ICP-MS and AFM, Biometals 27 (2014) 1337-1349.

DOI: 10.1007/s10534-014-9794-8

[4] J. Raff, U. Soltmann, S. Matys, S. Selenska-Pobell, H. Böttcher, W. Pompe, Biosorption of Uranium and Copper by Biocers, Chem. Mater. 15 (2003) 240-244.

DOI: 10.1021/cm021213l

[5] K. Pollmann, M. Merroun, J. Raff, C. Hennig, S. Selenska-Pobell, Manufacturing and characterization of Pd nanoparticles formed on immobilized bacterial cells, Lett. Appl. Microbiol. 43 (2006) 39–45.

DOI: 10.1111/j.1472-765x.2006.01919.x

[6] S. Dieluweit, D. Dieluweit, U.B. Pum, Sleytr, Formation of a gold superlattice on an S-layer with square lattice symmetry, Supramol. Sci. 5 (1998) 15-19.

DOI: 10.1016/s0968-5677(97)00073-4

[7] K. Fahmy, M. Merroun, K. Pollmann, J. Raff, O. Savchuk, C. Hennig, S. Selenska-Pobell, Secondary structure and Pd(II) coordination in S-Layer proteins from Bacillus sphaericus studied by infrared and X-ray absorption spectroscopy, Biophys. J. 91 (2006).

DOI: 10.1529/biophysj.105.079137

[8] D.M. Carrero, J.M. Morales, A.C. Garcia, N. Florez, P.A. Delgado, J. Dussan, A.C. Uribe, A.F.G. Barrios, Comparative analysis for three different immobilisation strategies in the hexavalent chromium biosorption process using Bacillus sphaericus S-layer, Can. J. Chem. Eng. 89 (2011).

DOI: 10.1002/cjce.20515

[9] J. Raff, K. Pollmann, T. Günther, A. Marquard, B. Katzschner, S. Matys and W. Pompe, Patent DE00102011006753B3 (2012).