The industrial development of the world has increased the demand of petroleum-based fuels sharply which are obtained from limited in certain regions of the world. As a result, most of the countries have to face energy crisis. Hence, it is necessary to look for alternative fuels which can be produced from resources available locally within the country. Lots of researches on biofuels such as alcohol, biodiesel and vegetable oils have been conducted to accelerate the development of a next generation of clean, green biofuels that can compete with fossil fuels in economics and well as performance. Biodiesel is a renewable, domestically produced fuel that has been shown to reduce particulate, hydrocarbon, and carbon monoxide emissions from combustion. In the present study an experimental investigation on emission characteristic of a liquid burner system operating on several percentage of biodiesel and gas oil is carried out. Samples of exhaust gas are analysed with Testo 350 Xl. The results show that biodiesel can lower some pollutant such as CO, CO2 and particulate matter emissions while NOx emission would increase in comparison with gas oil. They also demonstrate growth in temperature of exhaust gas with increase of percentage of biodiesel from B0 to B40. The results indicate there may be benefits to using biodiesel in industrial processes.