A Simple and Practicable PCR-Microchip with Bubble-Free Stationary Chamber

Jian Luo; Wei Ping Zhang; Wu Liu; Feng Cui; Wen Yuan Chen; Xiao Sheng Wu; Jing Tong Cao

doi:10.4028/www.scientific.net/AMM.483.51

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 483A Simple and Practicable PCR-Microchip with...

A Simple and Practicable PCR-Microchip with Bubble-Free Stationary Chamber

Abstract:

This paper reports on a simple and practicable biochip for polymerase chain reaction (PCR). The micro biochip (12mm×12mm) is a hybrid type which is composed of a polydimethylsiloxane (PDMS) cover with a hexagonal chamber and a glass substrate integrated with platinum (Pt) microheater and microsensor. Bubble formation has been reported as a big problem with PCR chips. In this paper we present a simple and practicable operation schedule to get a bubble free chip, in which a successful PCR process was guaranteed.

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Periodical:

Applied Mechanics and Materials (Volume 483)

Pages:

51-55

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.483.51

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Online since:

December 2013

Authors:

Jian Luo, Wei Ping Zhang, Wu Liu, Feng Cui, Wen Yuan Chen*, Xiao Sheng Wu, Jing Tong Cao

Keywords:

Bubble Free, Microchip, PCR, PDMS

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* - Corresponding Author

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