Study on the Radiosensitizing Applications of Gold Nanoparticles

Abstract:

Citrate-coated gold nanoparticles (AuNPs) were prepared by using redox method and its characterization was studied thoroughly. In the preparation process, the varieties of factors were examined by TEM morphology, DLS zetasizer, XPS analysis and UV-Vis spectroscopy for evaluating their qualities and stability. The experimental results obtained under optimal condition, which are 100±5°C of reaction temperature, 4.5-6.4 of the pH value, 2-10 of molar ratio between trisodium citrate and tetrachloroauric acid, respectively. The fluorescent intensities of DCFDA-stain indicate the amount of generated ROS (reactive oxygen species) by AuNPs. The cell cycle for MDA-MB-231 human breast cancer cells exhibits S-phase arrest which can prevent mitosis. The radiosensitizing effect of AuNPs with 20 nm diameter in MDA-MB-231 was investigated by RCGG values (ratio of cell numbers in the G2/M phase to the G0/G1 phase, RCGG) and its radiosensitizing efficiency was ca. 2.29 to 3.75 fold. In this study, it was found that the radiosensitizing efficiency is related to the cytotoxicity by AuNPs. After the irradiation by x-ray to cells, the enhancement value in the presence of 20 nm diameter AuNPs were 17.2% for 3 Gy and 18.9% for 7 Gy respectively. Compared to the irradiated group alone, the enhancements values were 21.8% and 19.7, respectively.

• Periodical: Applied Mechanics and Materials (Volume 851)
• Pages: 26-30
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.851.26
• Online since: August 2016
• Authors: Chang Shu Tsai*, Jou Kang Hsiao
• Keywords: Cell Cycle, Gold Nanoparticles, Radiosensitizer, RCGG Value, Redox Method

References

[1] A. Ito, H. Shinkai, H. Honda, et al. Heat-inducible TNF-αgene therapy combined with hyperthermia using magnetic nanoparticles as a novel tumor-targeted therapy, Cancer. Gene. Ther. 8(9) (2001) 649-654.

DOI: 10.1038/sj.cgt.7700357

[2] V. P. Zharov, K. E. Mercer, E. N. Galitovskaya, et al. Photothermal nanotherapeutics and nanodiagnostics for selective killing of bacteria targeted with gold nanoparticles, Biophys. J. 90(2) (2006) 619-627.

DOI: 10.1529/biophysj.105.061895

[3] J. D. Trono, K. Mizuno, N. Yusa, et al. Size, concentration and incubation time dependence of gold nanoparticle uptake into pancreas cancer cells and its future application to X-ray drug delivery system, J. Radiat. Res. 52(1) (2011) 103-109.

DOI: 10.1269/jrr.10068

[4] T. Kong, J. Zeng, X. Wang, et al. Enhancement of Radiation Cytotoxicity in Breast-Cancer Cells by Localized Attachment of Gold Nanoparticles, Small, 4(9) (2008) 1537-1543.

DOI: 10.1002/smll.200700794

[5] W. N. Rahman, N. Bishara, T. Ackerly, et al. Enhancement of radiation effects by gold nanoparticles for superficial radiation therapy, Nanomed. 5(2) (2009) 136-142.

DOI: 10.1016/j.nano.2009.01.014

[6] J. Petera, K. Odrazka, T. Frgala, et al. External beam radiotherapy and high-dose brachytherapy combined with cisplatin and paclitaxel in patients with advanced cervical carcinoma, Gynecol. Oncol. 99(2) (2005) 334-338.

DOI: 10.1016/j.ygyno.2005.06.015

[7] K. Katanyoo, S. Tangjitgamol, M. Chongthanakorn, et al. Treatment outcomes of concurrent weekly carboplatin with radiation therapy in locally advanced cervical cancer patients, Gynecol. Oncol. 123(3) (2011) 571-576.

DOI: 10.1016/j.ygyno.2011.09.001

[8] G. Frens, Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions, Nat. Phy. Sci. 241(105) (1973) 20-22.

DOI: 10.1038/physci241020a0

[9] M. K. Chow, C. F. Zukoski, Gold Sol Formation Mechanisms-Role of Colloidal Stability, J. Colloid Interf. Sci. 165(1) (1994) 97-109.

DOI: 10.1006/jcis.1994.1210

[10] S. K. Balasubramanian, L. Yang, L. Y. Yung, et al. Characterization, purification, and stability of gold nanoparticles, Biomater. 31(34) (2010) 9023-9030.

DOI: 10.1016/j.biomaterials.2010.08.012