A Development of Graphene Based Chemiresistive Sensor: Demonstrations on pH Sensing, and Cell Detection

Abstract:

Graphene is a single-to-few layer carbon sheet, consisting of carbon atoms hybridizing with one another via sp² configuration, providing outstanding mechanical, electrical, and electrochemical properties that can be utilized in various applications. In this work, we synthesized graphene powder via a modified Hummers method to obtain electrically insulating graphene oxide (GO), and converted the GO to semiconducting reduced graphene oxide (rGO) using L-ascorbic acid as a reducing agent. The rGO was re-dispersed in DI water and cast on prefabricated electrodes to create an rGO film chemiresistive sensor.The rGO sensor was shown to detect changes in buffer pH and cell concentrations of three different cells: human breast cancer cells (MDA-MB-231); non-small-cell lung cancer cells (A549); and fibroblast cells (L929). Sensor performances were determined in terms of "sensitivity", ratio of normalized resistance change upon exposure to a certain analyte concentration, and the analyte concentration. Our studies serve as strong evidence that the rGO-based chemiresistive sensor can be used for a quick and easy test in indicating buffer pH, or quantifying concentrations of a known cell.

• Periodical: Advanced Materials Research (Volume 1103)
• Pages: 137-143
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1103.137
• Online since: May 2015
• Authors: Sira Srinives*, Worasak Klongthong, Kanin Selamassakul, Navapass Peaunbida, Suchaporn Jiamjitton, Chinnawich Phamornnak, Chotiros Plabplueng, Bovornlak Oonkhanond, Tawatchai Charinpanitkul
• Keywords: Biosensor, Fibroblast, Graphene, Human Breast Cancer, Lung Cancer

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