Growth of a Methane-Utilizing Mixed Culture HD6T on Methanol and Poly-β-Hydroxybutyrate Biosynthesis
Methane-utilizing mixed culture HD6T was successfully cultivated in a brief non-sterile process using methanol as a sole carbon and energy source for the production of poly-β-hydroxybutyrate(PHB). Shake-flask experiments showed HD6T could grow well in the mineral salt medium with the addition of methanol exposed to the air directly. This non-sterile process and the use of cheap substrates (methanol) can reduce the production costs of PHB. It was found that HD6T grew better and PHB production in a more effective way with an initial liquid methanol concentration of 0.15%(v/v).The lag phase duration, the maximum growth rate, the biomass concentration and the PHB yield, for the optimal conditions were, respectively, 12.03h, 0.04h-1(OD600), 1.54g/l(dry weight), 0.424g/l(dry weight). Methane-utilizing mixed culture HD6T appears to be a promising organism for PHB production.
Guojun Zhang and Jessica Xu
J. Dong et al., "Growth of a Methane-Utilizing Mixed Culture HD6T on Methanol and Poly-β-Hydroxybutyrate Biosynthesis", Advanced Materials Research, Vols. 160-162, pp. 171-175, 2011