Growth of a Methane-Utilizing Mixed Culture HD6T on Methanol and Poly-β-Hydroxybutyrate Biosynthesis

Abstract:

Methane-utilizing mixed culture HD6T was successfully cultivated in a brief non-sterile process using methanol as a sole carbon and energy source for the production of poly-β-hydroxybutyrate(PHB). Shake-flask experiments showed HD6T could grow well in the mineral salt medium with the addition of methanol exposed to the air directly. This non-sterile process and the use of cheap substrates (methanol) can reduce the production costs of PHB. It was found that HD6T grew better and PHB production in a more effective way with an initial liquid methanol concentration of 0.15%(v/v).The lag phase duration, the maximum growth rate, the biomass concentration and the PHB yield, for the optimal conditions were, respectively, 12.03h, 0.04h-1(OD600), 1.54g/l(dry weight), 0.424g/l(dry weight). Methane-utilizing mixed culture HD6T appears to be a promising organism for PHB production.

• Periodical: Advanced Materials Research (Volumes 160-162)
• Pages: 171-175
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.160-162.171
• Online since: November 2010
• Authors: Jing Dong, Jia Ying Xin, Ying Xin Zhang, Lin Lin Chen, Hong Ye Liang, Chun Gu Xia
• Keywords: Cell Growth, Methane-Utilizing Mixed Culture Methanol, Poly-β-Hydroxybutyrate

References

[1] T. Yamane: Biotechnol. Bioeng. Vol. 41(1993), p.165.

[2] D. Bourque,B. Ouellette,G. Andr,D. Groleau: Appl. Microbiol. Biotechnol. Vol. 37(2000), p.7.

[3] J.A. Asenjo J.S. Suk,J. Ferment: Technol. Vol. 64(1986), p.271.

[4] O. Adegbola: Master of Science, Queen's University, Kingston, (2008).

[5] T. Wilkinson H.H. Topiwala and G. Hamer: Biotechnol. Bioeng. Vol. 16(1974), p.41.

[6] M. Tohyama,K. Shimizu: Eng J. Biochem. Vol. 4(1999), p.45.

[7] K.D. Wendlandt,M. Jechorek,J. Helm and U. Stottmeister: Polym. Degrad. Stabil. Vol. 59 (1998), p.191.

[8] Y.X. Zhang J.Y. Xin L.L. Chen, Ch.G. Xia: Appl. Biochem. Biotechnol. Vol. 157 (2009), p.431.

[9] R.L. Buchanan M.L. Cygnarowicz: Food Microbiology. Vol. 7(1990), p.237.

[10] J.C. Augustin,V. Carlier: International Journal of Food Microbiology. Vol. 56(2000), p.29.