Technological Optimization of Ethanol Fermentation on Energy Beet with Tolerance High Temperature Yeast Strain

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Abstract:

Temperature affected ethanol fermentation of energy beet greatly.This study showed that yeast HADY was tolerant to high temperature and when the fermentation temperature was 45°C,the translation rate of ethanol was higher.The three main factors were screened out from nine factors related fermentation by Plackett-Burman design and response surface methodology, such as substrate concentration, ratio of solvent to material, added HADY amount,nutrient salts, phosphorus addition amount, pH value, rotational speed, fermentation temperature and fermentation time. The three main factors included substrate concentration, fermentation temperature and phosphorus addition amount.The optimized technological parameters of HADY were as follows:substrate concentration12%, ratio of solvent to material 1:1, added HADY15%,nutrient salts 0.5,added phosphorus 0.9%, pH 5.0,rotation speed 130r/min, fermentation temperature 37°C and fermentation time 44 hours.At the time, the result showed that the translation rate of ethanol was 89.43%.

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Advanced Materials Research (Volumes 433-440)

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1245-1252

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January 2012

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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[1] Sun Yongming, Yuan Zhenhong, Sun Zhenjun. Status and future of bioenergy and biomass utilization[J]. Renewable Energy, 2006, (2): 78-82.

Google Scholar

[2] Sun Zhenjun . Biomass industry and its developmental trends in China[J]. Transactions of the CSAE, 2004, 20(5): 1-5.

Google Scholar

[3] Zhou Zhongren, Wu Wenliang. Status quo and prospects of biomass enerny[J]. Transactions of the CSAE, 2005, 21(12): 12-15.

Google Scholar

[4] Yan Liangzheng, Zhang Lin, Wang Shiqiang, et al. Potential yields of bio-ethanol from energy crops and their regional distribution in China[J]. Transactions of the CSAE, 2008, 24(5): 213-216.

Google Scholar

[5] Yan Lin, Shuzo Tanaka. Ethanol fermentation from biomass resources: current state and prospects[J]. Appl Microbiol Biotechnol, 2006, 69: 627-642.

DOI: 10.1007/s00253-005-0229-x

Google Scholar

[6] Bai F W, Anderson W A, Moo-Young M. Ethanol fermentation technologies from sugar and starch feedstocks[J]. Bioechnology Advances, 2008, 26: 89-10.

DOI: 10.1016/j.biotechadv.2007.09.002

Google Scholar

[7] Gi-Wook Choi, Hyun-Woo Kang , Se-Kwon Moon. Repeated-batch fermentation using flocculent hybrid, Saccharomyces cerevisiae CHFY0321 for efficient production of bioethanol[J]. Applied Microbiology and Biotechnology, 2009, 84(2): 261-269.

DOI: 10.1007/s00253-009-1946-3

Google Scholar

[8] Hussy I, Hawkes F R, Dinsdale R, et al. Continuous fermentative hydrogen production from sucrose and sugarbeet[J]. Hydrogen Energy,2005,30:471-483.

DOI: 10.1016/j.ijhydene.2004.04.003

Google Scholar

[9] Yue Guojun, Wu Guoqing, Hao Xiaoming. The status quo and prospects of fuel ethanol process technology in China[J]. Progress in Chemistry, 2007, 19(7): 1084-1090.

Google Scholar

[10] Song Yongfang. Progress of exploitation and utilization on energy plant[J]. Biomass Chemical Engineering, 2006, 40(6): 51-53.

Google Scholar

[11] Shi Shuzhi, Cheng Dayou, Ma Fengming, et al. Exploitation and utilization of biomass energy crop-energy beet[J]. Chinese Agricultural Science Bulletin,2007,(11): 416-419.

Google Scholar

[12] Song Xinlei, Zhang Peng. Effects on the fermentation property of the strain in ethanol fermentation of xylose[J]. LIQUOR MAKING, 2007, 34(1): 40-43.

Google Scholar

[13] Jin Hui, Liu Ronghou. Effects of different temperatures on ethanol fermentation of Sweet Sorghum stalk juice[J]. Journal of Anhui Agri. Soi. 2007, 35(19): 5684-5685.

Google Scholar

[14] Liu Chang, Wang Tao, Shi Cuifang. Study on screening and characteristics of thermotolerant yeast[J]. LIQUOR MAKING, 2007, 34(2): 52-54.

Google Scholar

[15] Wu Weixiang, Min Hang, Kamdem DGC. Biological and fermentative properties of a Thermotolerant yeast Strain in alcohol production from cassava flour. Journal of Zhejiang University (Agric. & Life Sci. ), 2001, 27(3): 277-281.

Google Scholar

[16] Shi Shuzhi, Cheng Dayou, Xu Dechang, et al. Technological optimization and mathematical model of alcohol fermentation of energy beet[J]. Transactions of the CSAE, 2009, 25(6): 214-218.

Google Scholar

[17] Antonio De León-Rodríguez, Pilar Escalante-Minakata, Ana P. Barba de la Rosa, et al. Optimization of fermentation conditions for the production of the mescal from Agave salmiana using response surface methodology[J]. Chemical Engineering and Processing, 2008, 47: 76-82.

DOI: 10.1016/j.cep.2007.08.010

Google Scholar

[18] Ning Guo, Fang Gong, Zhenming Chi, et al. Enhanced inulinase production in solid state fermentation by a mutant of the marine yeast Pichia guilliermondii using surface response methodology and inulin hydrolysis [J]. Microbiol Biotechnol , 2009, 36: 499–507.

DOI: 10.1007/s10295-008-0519-2

Google Scholar