Advanced Materials Research Vols. 554-556

Abstract: To study the relation between α-actinin1 gene and reproduction traits, 442 different varieties cattle were used as experimental populations. PCR-RFLP and DNA sequencing was used to detect the SNP and genotype analysis in these populations. RT-PCR technique was used to isolate cDNA of bovine α-actinin1gene. The association analysis of this ApaⅠ locus polymorphism with reproduction traits were analyzed by using GLM procedure. The result showed that the mutation of G/A,using ApaⅠ endonuclease, was detected in 10th intron of at the α-actinin1 gene locus. The mutation caused ApaⅠpolymorphism in group, there were α-actinin1-G and α-actinin1-A genotypes.The statistical analysis of α-actinin1 163-SNP with reproduction traits showed that there was no associated with the number of calving.The sequence of cDNA of bovine α-actinin1 was 2 911bp. It contained an open reading frame of 2 679 bases (from 53bp to 2 731bp) which encoded an 892 amino acid.

Abstract: Leaf dipping method is used in this paper to compare the drug resistance of the invasive species western flower thrips and the native thrips tabaci to 6 kinds of insecticides: phoxim, chlorpyrifos, beta-cypermethrin, spinosad, emamectin-benzoate and avermectin.The results showed that the western flower thrips had a higher drug resistance to phoxim, chlorpyrifos and beta-cypermethrin than thrips tabaci, while beta-cypermethrin and avermectin had a good activity against thrips tabaci. Spinosad had the best control effect, with a concentration of 0.5 mg/L, the corrected mortality of these two thrips reached above 90%.

Abstract: To exploit sensitive, accurate and convenient detection techniques for nucleic acid sequences can help to overcome the defects of current fluorescence detection method. Raman spectroscopy represents one family of methods that may offer such potential. In this paper, G•C base pairs and C•U mispairs were studied by Raman spectral characterization of single-strand homopolynucleotides, i.e. polyguanylic acid (PolyG), polycytidylic acid (PolyC) and polyuridylic acid (PolyU), and their equimolar complexes PolyG•PolyC and PolyC•PolyU in solution of 0.14 M Na⁺, pH7.0. Both of the complexes were found to adopt A type conformation. Helix formation between complementary polynucleotides led to marked and characterisctic changes in frequency and intensity of Raman bands of the biopolymers. Spectral changes were also observed upon the formation of C•U mispairs. According to the Raman data, in C•U mispairs the C2=O of U was engaged in a hydrogen bond to the N4H₂ group of cytosine, while the uracil C4=O group is free. Some of the rC residues transformed from C3-endo/anti to C2-endo/anti conformation due to base mispairing.The results suggest that Raman spectroscopy provides detailed and highly sensitive information about both the structure of nucleic acids and their base composition, and has great potential for analysis of DNA sequences.

Abstract: The intestinal-lysis and multi-probiotics microcapsule of L.lactis, L.plantarum and B.bifidum was produced with gelatin and chitosan as wall material by emulsified condensation method. The effects of temperature and the artificial simulation gastric and intestinal juices on the activity of probiotics were investigated. The experimental results show that the storage time of the bacteria was prolonged and the survival rates of the bacteria in the stomach and the intestine were increased by the formation of microcapsule to embed the bacteria, which can play a target action and reduce the production cost.

Abstract: Hovenia dulcis Thunb, a food supplement and traditional medicine used in East Asia, has been demonstrated to exhibit hepatoprotective, antimicrobial, neuroprotective, and anti-oxidant activities. To evaluate the anti-oxidant activities of H.dulcis in STZ-induced diabetic mice, the different extracting solvents were employed to compare the antioxidant activity by the classical Fenton’s reagent, the concentrations of SOD, MDA, and iNOS in the serum were determined. The results showed that the ethanol (70%) exhibited the most effective antioxidant activity and dose-dependently upregulation of SOD and downregulation of MDA and iNOS significantly in STZ-induced diabetic mice. These indicated that H.dulcis displayed remarkable anti-diabetic activity.

Abstract: The binding reaction of nickel(II) complex [Ni(C₁₆H₂₀N₂)₂•(H₂O)₂]Cl₂•C₃H₇NO with bovine serum albumin(BSA) was studied by fluorescence spectroscopy under the simulative physiological conditions. The experimental results show that the fluorescence quenching of BSA by nickel(II) complex is a result of the formation of ground state complex and the quenching mechanism was static quenching. The binding constants were 4.24×10³ L•mol⁻¹ at 293K with one binding site. The antimicrobial activity study found that the nickel(II) complex was active against Escherichia coli, Staphylococcus aureus and Bacillus subtilis.

Abstract: Squalene, a natural antioxidant, was microencapsulated by the method of ultrasonic spraying and freeze-drying using shell materials of gelatin and arabic gum. The suitable condition for ultrasonic emulsification before ultrasonic spraying and freeze-drying was frequency 28 kHz and running time 20 min. The reasonable condition for microencapsulation of squalene by ultrasonic spraying and freeze-drying was gelatin 3.0 %, arabic gum 5.0 %, maltodextrin 6.0 % and sucrose ester 0.9 %. The appearance and size of microcapsules of squalene were measured by scanning electron microscope and metallographic microscope. The microcapsules of squalene were smonth spheres at the diameter of about 10 μm.

Abstract: Using methacrylic acid (MAA) as monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker and phentolamine as template molecule, we synthesized phentolamine–molecularly imprinted polymers (MIP). Using phentolamine–MIP as molecule recognizates, based on the oxidation reaction between KMnO₄ and phenolamine in acidic, we set up a flow-injection chemiluminescence (FI-CL) sensor for phentolamine determination. The sensor was fabricated by packing the MIP particles into a V-shape flow cell. Phentolamine could be selectively on-line adsorbed by the MIPs and then sensed by the oxidation reaction between KMnO₄ and phenolamine in acidic.The CL intensity was linear to phentolamine concentration in the range from 2.0 ×10−8 to 6.0 ×10−6 g mL−1 and the limit of detection (L.O.D.) was 6.0 ×10−9 g mL−1 (3σ) with the relative standard deviation (R.S.D.) less than 5% (n=11). The sensor showed high selectivity, improved sensitivity and excellent stability and reversibility compared with the CL method without using MIPs. The lifetime of the sensor was investigated and MIPs could be reused for more than 200 times. Using the method to determine phentolamine in human urine, the recoveries of phentolamine is 100.0% to 104.0%, the result is pretty good.

Abstract: Lagotis yunnanensis is a folkloric medicine in China. In order to investigate its effective components, The 95% ethanol extract of whole plant of L. yunnanensis was separated and purified with chromatographic method. Eight compounds were obtained and identified as luteolin (1), chrysoeriol (2), apigenin-7-methyl ether (3), 3, 4-dimethoxycinnamyl-D-glucopyranoside (4), verbascoside (5), cistanoside D (6), 2-O--D-glucopyranospylcucurbitacin D (7) and arvenin I (8) by spectroscopic methods. Compounds 1~3 are flavonoids, compounds 4~6 are phenylpropanoids and compounds 7 and 8 are triterpenoids. Compounds 4~8 have been obtained from this species for the first time.

Abstract: More new phenoxypropylamines have been synthesized. All the products have been characterized by elemental analysis, 1H-NMR, ¹C-NMR and MS. The biological activity effects of the title compounds were examined. From the biological activity re^{Superscript text}sults, we found that some of them showed significant gastric acid antisecretory activity.