Advanced Materials Research Vol. 643

Abstract: A simple, sensitive and quick assay to simultaneously determine lomefloxacin (LFLX) and ciprofloxacin (CPLX) had been developed by using zero-crossing first derivative constant wavelength synchronous spectrofluorimetry. Due to their similar molecule structures, it was difficult to analysis and determine LFLX and CPLX simultaneously by conventional fluorometry. In order to improve the sensitivity, aluminium ion was used to significantly enhance their endogenous fluorescent intensity. The different experimental parameters affecting the synchronous fluorescence intensity of the two fluoroquinolone drugs were carefully studied and optimized. The proposed method was successfully applied for the determination of the two drugs in milk samples.

Abstract: Although we have gained much information about lead-induced organ damage, the effect of blood lead level on T cell subgroup is yet to be determined. To assess the effect of blood lead level on T-lymphocyte Subpopulation Expression of children, and the association of T-lymphocyte Subpopulation Expression with threshold limit value of blood lead level.The aim of study is evaluating the significance of blood level, as an indicator for environmental lead, and T-lymphocyte Subpopulation Expression as an effect indicator, to determinate the correlation between the content of blood lead and T-lymphocyte Subpopulation Expression. The 120 children from area to exposure environmental lead were recruited into the study using immunofluorescence methods and study of blood lead level using graphite stove atom absorption spectrophotometer respectively, with blood lead level of 0.48μmol/L as a cut off value. The enrolled children according to their blood lead levels were assigned into three groups,26 in Group I with blood lead level ≥0.48 μmol/L , 40 in Group 2 with lead level ≥0.24 μmol/L but <0.48 μmol/L and 54 in Group 3 with lead level <0.24 μmol/L .Student t test was used in data analysis, and correlation analysis was used to assess the correlation between blood lead level and expression of T-lymphocyte Subpopulation.Data from all 120 children were used for data analysis. There was no significant difference when blood lead level of 0.24μmol/L as a cut off value. Our analysis of CD3, CD4 cells expression and CD4/CD8 cell ratio decreased in high lead groups of 0.48μmol/L as a cut off value(lead level ≥0.48 μmol/L) than Group (lead level ≥0.24 μmol/L) (t=3.27,P<0.01). When the blood lead level was ≥0.48 μmol/L, The result revealed the level of T-lymphocyte Subpopulation expression was a significant difference between groups of high and low blood lead level.. There was difference correlation in the level of blood lead and T-lymphocyte Subpopulation expression. The result suggested that the high blood lead level may be regarded as an adverse effect on children’s immune function especially on CD3 percentage, CD4/CD8 cell ratio when exposed environmental lead.

Abstract: The squid gelati were extracted by enzymatic degradation method. and Chuanxiong polysaccharide were prepared by water extract-alcohol method. 0.1% chuanxiong polysaccharide solution and 2% squid skin collagen solution were mixed, and cross-linked. A novel scaffold, the squid gelatin-polysaccharide scaffold (SGPS), was fabricated from the squid gelatin and Chuanxiong polysaccharide using glutaraldehyde as a crosslinking agent by lyophilizing.

Abstract: The sequences of Potato α-Amylase Gene amyA2 was analysised by Bioinformatics, including its codon usage bias, physical and chemical properties, subcellular localization, and conserved structures. The results showed that the cDNA had a 1218 bp open reading frame and was referred to as amyA2, which encodes for an α-amylase with 405 amino acid residues (GenBank accession number: GU134783), and shared 98% identity with a published potato α-amylase (GenBank accession number: M79328.1) at the amino acid level. The amino sequences contains a catalytic domain (PF00128、SM00624) between 20 to 348 and a C-terminal beta-sheet domain between 349-407, which are similar to ones of the amylase family 13. Eight-stranded alpha/beta barrel was also found in the enzyme, which was thought as an active site of α-amylase.

Abstract: Two new europium (III) complexes luminescent materials, Eu-PDA and Eu-PDA-Phen, were prepared by the reaction of Eu(NO3)3·6H2O with 2, 3-pyrazinedicarboxylic acid (PDA) and 1,10-phenanthroline, respectively. The luminescent properties of two europium (III) complexes in solid and in organic solvents have been studied. The effect of organic solvents and metal ions (Ca2+ and Mg2+) on the luminescent intensity of the Eu (III) complexes have also been investigated. The results show that the metal ions (Ca2+ and Mg2+) could enhance the luminescent intensity of Eu-PDA-Phen complex and reduce luminescent intensity of Eu-PDA complex. The polarity of organic solvents is an important factor on the luminescent intensity of two complexes.

Abstract: Light green crystals of the Ni (II) complex material, [Ni(H2O)6](C14H16N2O6S2), were obtained by the self-assembly of Ni(H2O)6·6H2O, 4-amino-3-methyl-benzenesulfonic acid and NaOH. The result of X-ray diffraction shows that each Ni (II) atom is six-coordinated in a distorted octahedral environment by six oxygen atoms from the water molecules. The complex molecules are connected by hydrogen bonds to form two dimensional network structure. The thermal analysis shows that the complex has a enothermic peak at 302 C, indicating that coordinated water molecules are in the complex.

Abstract: The development of tissue engineering techniques for cartilage repair and regeneration provided a new way, according to their own characteristics and structure of the cartilage, as artificial cartilage replacement materials and scaffold materials should have good biomechanical properties. The effect of autologous cartilage transplantation is best in the field of articular cartilage repair, the study of bone marrow stromal cells in vitro tests and animal experiments was more, and the clinical application was less, which is still in the stage of exploration. Biomaterials material for tissue repair plays a more and more important role, especially in athletic injury.

Abstract: Spindle-like hollow mesoporous silica nanoparticles (spindle-like HMSNs) were fabricated via the template-sacrificial technique using hematite (Fe₂O₃) spindles as hard template, tetraethylorthosilicate (TEOS) as precursor and cetyltrimethyl ammonium bromide (CTAB) as the pore directing agent. The Fe₂O₃ core and CTAB were selectively removed by hydrochoric acid aqueous solution etching. Transmission electron microscopy results revealed that spindle-like HMSNs with thickness of shell range from 8 to 47 nm were prepared by changing the concentration of the reactant. This methodology provides a facile and robust route for preparation of hollow mesoporous materials.

Abstract: Mesoporous CeO2 was first synthesized by hydrothermal method, and then used to synthesize different content of (Co3O4)x/CeO2 (x was the molar ratio of Cu and Co) by deposition-precipitation method. The fresh and doped catalysts were characterized by X-ray diffraction (XRD), N2 adsorption and desorption, H2 temperature programmed reduction (H2-TPR) and O2 temperature programmed desorption (O2-TPD) to study the crystal structure, surface area, and the mechanism of CO oxidation. The results show that: In XRD pattems, the doped cobalt amounts of samples from x=20% to x=100% have Co3O4 crystal structure. The N2 adsorption and desorption indicated the samples were mesoporous structure. Compared with other samples, the better reducibility and activity oxygen species of (Co3O4)50%/CeO2 coincided with its better catalytic activity.

Abstract: A novel thermoresponsive Cu(II) ion-imprinted hydrogel [Cu(II)-IIH] with interpenetrating network structure has been prepared to remove Cu(II) ions with high selectivity. The Cu(II)-IIH was prepared by free radical/cationic hybrid photopolymerization of N-isopropylacrylamide (NIPAM) and triethylene glycol divinyl ether (DVE-3) using Cu(II) ion as template. The Cu(II)-IIH was used to remove heavy metal ions from aqueous solution at 20 and 40oC. The Cu(II)-IIH selectively bound the template ion above a lower critical solution temperature (LCST) of poly(N-isopropylacrylamide) (PNIPAM). The memory was fixed by shrinking above the LCST, and was deleted by swelling below the LCST. Selective adsorption study indicated that ion imprinting resulted in stronger affinity of the Cu(II)-IIH toward Cu(II) ions than other competitor metal ions compared with the non-imprinted hydrogel (NIH).