Experimental Study of the Biochemistry Method for Enhancing Oil Recovery in the Oil Reservoir after Polymer Flooding

Abstract:

A delayed cross linked gel profile control agent is used to plug high permeable formations. Also, well nutrient fluid and microbes are injected with 50% of the heavy residual oil, after polymer flooding, in order to improve oil recovery due to the complex environment of oil reservoirs. Four strains of polycyclic aromatic hydrocarbon-degrading bacteria are selected from oilfield produced water with a high efficiency. Two of the four strains, namely BISYX17 and BISYX14, are new. Polycyclic aromatic hydrocarbon-degrading bacteria have high growth activity and they are able to reach a maximum stain concentration after being cultured 4 to 8 days, using phenanthrene as their sole carbon source. They are able to effectively degrade heavy hydrocarbon with a phenanthrene degradation rate of up to 80％, after the sample is cultured for seven days. Strain BISYX7 has the strongest phenanthrene -degrading ability, with a maximum degradation percentage of 89.89％. The strains are capable of producing dioxygenase to open rings of polycyclic aromatic hydrocarbon. The dioxygenase activity, produced by BISYX17, is able to reach 40.2 IU/mg, which is higher than the enzyme activities of a wild strain. This shows the strain has excellent potential to produce enzymes. Enzymes, produced by metabolism, have a direct degradation rate of 68% on crude oil. A core displacement simulation experiment indicates a profile control oil-displacing system is able to improve crude oil recovery efficiency by 17%, after polymer flooding. Thus, the system has excellent application potential for residual oil recovery.

• Periodical: Advanced Materials Research (Volume 647)
• Pages: 144-149
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.647.144
• Online since: January 2013
• Authors: Yue Hui She, Fan Zhang, Bo Xun Liang, Zheng Liang Wang, Long Jiang Yu
• Keywords: Enhanced Oil Recovery (EOR), Gel Profile Control Agent, Heavy Hydrocarbon-Degrading Bacteria, Polymer Flooding

References

[1] Yi Shaojin, She Yuehui. Petroleum and environmental microorganism technology, China university of geosciences press, 2002，P64-88.

[2] Wang Hui et a1．(2003). The summarization of microbial enhanced oil recovery technology. Petroleum geology and oilfield development in Daqing, V22（5）, P49—52.

[3] Hao Geiyu et a1．(2004). Microbial enhanced oil recovery technology and developing trends, Energy environment protection, V18（2）, P8—13.

[4] Jonathan D.V.H., Ajay S. and Owen P.W. (2003)Recent Advances in Petroleum Microbiology. Microbiology and Molecular Biology Reviews. V67(4), P503-549.

[5] Lei Guanglun, Guo Yunyao, Deng Jiapeng et a1．Laboratory research of improving oil recovery by microbe at high temperature [J]. Petroleum geology and recovery efficiency, 2002，9(4): 15-20.

[6] Luo Ruxin, Zhang Sunqin, Li Shunpeng. Location, cloning and expression of catechol 1, 2-dioxygenase (C120) gene. Chinese journal of applied and environmental biology, 1999，5（2）：208-211.

[7] Zhang Jie, Liu Yongsheng, Feng Jiaxun et a1．Clong, location and over expression of catechol 2, 3-dioxygenase gene, Chinese journal of applied and environmental biology, 2003，9（5）：542-545.

[8] Liu, J. F., Ma, L. J., Mu, B. Z., Liu, R. L., Ni, F. T., & Zhou, J. X. (2005). The field pilot of microbial enhanced oil recovery in a high temperature petroleum reservoir. Journal of Petroleum Science and Engineering, 48, 265–271.

DOI: 10.1016/j.petrol.2005.06.008

[9] Ilker Uz, Y. P. Duan and A. Ogram. Characterization of the naphthalene-degrading bacterium, Rhodococcus opacus M213. FEMS Microbiology Letters, Volume 185, Issue 2, 15 April 2000, Pages 231-238.

DOI: 10.1111/j.1574-6968.2000.tb09067.x

[10] Fabrizio Briganti, Enrica Pessione, Carlo Giunta and Andrea Scozzafava. Purification, biochemical properties and substrate specificity of a catechol 1, 2-dioxygenase from a phenol degrading Acinetobacter radioresistens . FEBS Letters, Volume 416, Issue 1, 13 October 1997, Pages 61-64.

DOI: 10.1016/s0014-5793(97)01167-8

[11] Jangho Moon, Kyung Rak Min, Chi-Kyung Kim, Kyung-Hee Min and Youngsoo Kim. Characterization of the Gene Encoding Catechol 2, 3-Dioxygenase ofAlcaligenessp. KF711: Overexpression, Enzyme Purification, and Nucleotide Sequencing. Archives of Biochemistry and Biophysics, Volume 332, Issue 2, 15 August 1996, Pages 248-254.

DOI: 10.1006/abbi.1996.0339