Amperometric Biosensor for Hydrogen Peroxide Based on Glucose Oxidase Immobilized in Au Nanoparticles and 2,3-Dimercaptosuccinic Acid Self-Assembly Monolayers

Abstract:

A simple amperometric biosensor for the analytical detection of hydrogen peroxide was developed. The biosensor was prepared by the immobilization of glucose oxidase (GOD) in the nanostructure matrix constructed through electrochemical deposition of Au nanoparticles (AuNPs) upon 2, 3-dimercaptosuccinic acid (DMSA) SAMs formed on Au electrode. The fabricated biosensor had a fast response of H₂O₂ (<10 s), and an excellent linear range of concentration from 2×10⁶ to 3.5×10³ M with a low detection limit of 0.65 μM (S/N = 3) under the optimum conditions. The Michaelis-Menten constant of the immobilized GOD was 2.0 mM, displaying a high affinity of the GOD toward H₂O₂ without loss of enzymatic activity in nanosubstrate.

• Periodical: Advanced Materials Research (Volume 704)
• Pages: 252-257
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.704.252
• Online since: June 2013
• Authors: Zhao Hao Wang, Yan Li Shang, Qi Jin Wan, Nian Jun Yang
• Keywords: Au Nanoparticles, Glucose Oxidase, H₂O₂ Biosensors, Self-Assembled Monolayers (SAMs), Three-Dimensional Electrodes

References

[1] X. Shu, Y. Chen, H. Yuan, S. Gao, D. Xiao, H2O2 sensor based on the room-temperature phosphorescence of Nano TiO2/SiO2 composite, Anal. Chem. 79 (2007) 3695-3702.

DOI: 10.1021/ac0624142

[2] J. Jia, B. Wang, A. Wu, G. Cheng, Z. Li, S. Dong, A method to construct a third-generation horseradish peroxidase biosensor: self-assembling gold nanoparticles to three-dimensional sol-gel network, Anal. Chem. 74 (2002) 2217-2223.

DOI: 10.1021/ac011116w

[3] Y. Liu, F. Yin, Y. Long, Z. Zhang, S. Yao, Study of the immobilization of alcohol dehydrogenase on Au-colloid modified gold electrode by piezoelectric quartz crystal sensor, cyclic voltammetry, and electrochemical impedance techniques, J. Colloid Interface Sci. 258 (2003) 75-81.

DOI: 10.1016/s0021-9797(02)00083-8

[4] A. Gole, C. Ganpule, R. Pasricha, M. Sastry, Quasi-linear assemblies of silver nanoparticles by highly localized anodic dissolution of copper in the hydrosol, J. Nanosci. Nanotechnol. 2(2002) 147-150.

DOI: 10.1166/jnn.2002.106

[5] H. Yin, S. Ai, W. Shi, L. Zhu, A novel hydrogen peroxide biosensor based on horseradish peroxidase immobilized on gold nanoparticles-silk fibroin modified glassy carbon electrode and direct electrochemistry of horseradish peroxidase, Sens. Actuators, B. 137 (2009) 747-753.

DOI: 10.1016/j.snb.2008.12.046

[6] A.K. Upadhyay, T.W. Ting, S.M. Chen, Amperometric biosensor for hydrogen peroxide based on coimmobilized horseradish peroxidase and methylene green in ormosils matrix with multiwalled carbon nanotubes, Talanta. 79 (2009) 38-45.

DOI: 10.1016/j.talanta.2009.03.010

[7] D.K. Peng, S.T. Yu, D.J. Alberts, J. Lahann, Switching the electrochemical impedance of low-density self-assembled monolayers, Langmuir. 23 (2007) 297-304.

DOI: 10.1021/la061677o