A Simple Hydrogen Peroxide Biosensor Based on Catalase Immobilized in Nano-Structural Composite Film

Abstract:

A robust and effective nanocomposite film modified gold electrode based on 2, 3-dimercaptosuccinic acid (DMSA) and Au nanoparticles (AuNPs) was prepared by a method combining self-assembly with underpotential deposition. Direct electron transfer can easily take place between a gold electrode and catalase molecules anchored on AuNPs films. Besides, the CAT/AuNPs/DMSA multilayer film had a relatively rapid and satisfactory response toward H₂O₂ with a wide linear range from 3.0×10⁶ to 5.86×10³ M and a low detection limit of 3.0 μM (S/N = 3). The Michaelis-Menten constant of the immobilized CAT was calculated to be 2.2 mM. The simple CAT/AuNPs/DMSA/Au system can be developed into a H₂O₂ biosensor.

• Periodical: Advanced Materials Research (Volume 704)
• Pages: 258-263
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.704.258
• Online since: June 2013
• Authors: Zhao Hao Wang, Han Cui, Qi Jin Wan, Nian Jun Yang
• Keywords: Au Nanoparticles, Catalase, H₂O₂ Biosensors, Self-Assembled Monolayers (SAMs)

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