Immobilization of β-Glucosidase on Modified Attapulgite

Jianbo Jia; Yong Yang

doi:10.4028/www.scientific.net/AMR.774-776.540

Paper Titles

Magnetic States of Nanoparticles with RKKY Interaction
p.523

Tests on Effect of Gravity and Soil Density on Soil Cone Index
p.528

Preparation and Characterization of Magnetic Graphene Oxide
p.532

Mesoporous Silica Nanoparticles with Controllable Pore Size: Preparation and Drug Release
p.536

Immobilization of β-Glucosidase on Modified Attapulgite
p.540

Water-Swelling Rubber Containing Small Amount of Nanofillers with Enhanced Water Swelling Durability
p.544

Failure Mechanisms of Nanoparticle Reinforced Metal Matrix Composite
p.548

A Study on the Start-up Period of Underground Infiltration System Treating Rural Domestic Sewage
p.552

A Review of Ferrates (VI) Oxidation of Organic Compounds
p.556

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 774-776Immobilization of β-Glucosidase on Modified...

Immobilization of β-Glucosidase on Modified Attapulgite

Abstract:

Attapulgite was modified with different methods and used as support to immobilize β-glucosidase. Effects of enzyme dosage, pH, temperature and time in immobilization on stabilities of immobilized β-glucosidase were investigated. The results showed that the optimum immobilization conditions of β-glucosidase on organic modification attapulgite were as follows: enzyme dosage 3600 U/g, temperature 50 °C, time 60 min and pH 5.0. The thermal and storage stabilities of immobilized β-glucosidase were improved significantly.

You might also be interested in these eBooks

Advanced Technologies in Manufacturing, Engineering and Materials

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 774-776)

Pages:

540-543

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.774-776.540

Citation:

Cite this paper

Online since:

September 2013

Authors:

Jianbo Jia, Yong Yang

Keywords:

4-nitrophenyl-β-D-glucopyranoside, Attapulgite, Immobilisation, Organic Modification, β-Glucosidase

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] Callanan M J, Russell W M and Klaenhammer T R, Modification of lactobacillus β-glucosidase activity by random mutagenesis, Gene. 389(2007) 122-127.

DOI: 10.1016/j.gene.2006.10.022

Google Scholar

[2] Luo G M, Enzyme Engineering, Press of Chemical Industry, Beijing, 2004, pp.55-76.

Google Scholar

[3] Qian Yunhua, Jin Yeling and Chen Jing, Study on Preparing Superfine Powder & Surface Modification of Attapulgite & Its Application, Non-metallic Mines. 30(2009) 9-11.

Google Scholar

[4] Frost R L, Mendelovici E, Modification of fibrous silicates surfaces with organic derivatives: an infrared spectroscopic study, Journal of Colloid and Interface Science, 294(2006) 47-52.

DOI: 10.1016/j.jcis.2005.07.014

Google Scholar

[5] Lei Z Q and Wen S X, Preparation and decoloration property of polystyrene-grafted palygorskite nanoparticles, Materials Letters, 61(2007) 4076-4078.

DOI: 10.1016/j.matlet.2007.01.041

Google Scholar

[6] Yongzheng Pan, Yue Xu, Li An, et al., Hybrid network structure and mechanical properties of rodlike silicate/cyanate ester nanocomposite, Macromolecules, 41(2008) 9245-9258.

DOI: 10.1021/ma800819s

Google Scholar

[7] Li P, Wan X C and Ding X L, Determine and Characteristics of β-glucosidase from Aspergillus niger, Journal of Anhui Agricultural University, 25(1998) 304-309.

Google Scholar