Modulation of Fibrin Gel Extracellular Matrix Properties by Fibrinogen and Thrombin Concentrations for Angiogenesis Assay

Abstract:

Angiogenesis is the formation of new microvascular network from the pre-existing blood vessel. In tissue engineering approaches, angiogenesis is essential for the promotion of micro-vascular network inside an engineered scaffold construct, mimicking a functional blood vessel in vivo. In the in vivo system, the formation of new blood vessels depends on the properties fibrin gel extracellular matrix. In this study, we have investigated the effect of different fibrinogen and thrombin composition on the biophysical properties of fibrin gel. Higher concentration of thrombin (4.0 Units/milliliter) yields a shorter clotting time of the fibrin gel and result in better water uptake property while at lower concentration of thrombin (0.5 Units/milliliter), the clotting time takes much longer. Also, at lowest concentration ratio of fibrinogen to thrombin (0.5 milligram/milliliter to 4.0 Units/milliliter), the turbidity study shows the lowest absorbance compared to other samples. Different concentration of fibrinogen and thrombin also affect the microstructure of the fibrin gel. The variation of these properties will be then manipulated to be used for in vitro angiogenesis. This study opens broader application of fibrin extracellular matrix in regenerative medicine and tissue engineering researches.

• Periodical: Advanced Materials Research (Volume 911)
• Pages: 342-346
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.911.342
• Online since: March 2014
• Authors: Siti Amirah Ishak*, Irza Sukmana
• Keywords: Angiogenesis Assay, Biophysical Properties, Extracellular Matrix, Fibrin Gel

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