Advanced Materials Research Vols. 912-914

Abstract: This paper presents the results of three different fibers, Kenaf (hibiscus cannabinus), oil palm (Elaeis guineensis) and abaca (Musa textilis) which were treated using 5 different types of surface treatments namely sodium hydroxide for 24 hours, glycidoxypropyltrimethoxy-silane for 24 hours, NaOH followed by Silane (each 24 hours), NaOH followed by Silane (each 12 hours) and NaOH followed by Silane (each 6 hours). The mechanical strength of the fibers were then studied and compared to the untreated fibers. The highest strength was given by the fibers treated with NaOH followed by the combined treatments and the lowest were the fibers treated with silane. Also the bonding strength between the fibers and unsaturated polyester resin were then evaluated using micro droplet pull out tests. It was proven that all the treatments improved on the bonding strength. The highest was silane followed by NaOH. For the combined treatments it was found that the highest IFSS was given by the lowest treatment duration at 6 hours, due to the alkali nature of NaOH corroding the fibers which were then filled with silane, thus losing the ability to properly bond with the resin. The samples were also then characterized using a SEM to check the surface morphology which revealed that the NaOH reduced the diameter by removing impurities and lignin, thus increasing the aspect ratio. While silane coats the surface, increases the diameter and reduces the aspect ratio. Keywords. Kenaf, Abaca, OPF, Chemical Treatment, Bonding Strength, Droplet Test, SEM

Abstract: To evaluate the control effect of Oviductus ranae on liver fibrosis in rats, and the change of TGF-β and α-SMA in liver of. To explore the mechanism of Oviductus ranae decoction on liver fibrosis. Methods Wistar female rats were randomly divided into a blank control group, model control group, colchicines group, Oviductus ranae group. Using the CCl₄ composite approach to make the rat modle. The course of treat-mart was 12 weeks.After treatment,All the rats was killed,and the materials and blood was taken,and to detect biochemical test of liver function after eight weeks. Investigating the variation of liver histology. Meanwhile detecting protein expression of TGF-β and α-SMA and by immunehistochemical method.Result The general condition of rats in all treatment groups are worse than the blank group,but better than the model group. And the rats in the model group were all occurred in liver fibrosis,and liver fibrosis is the most serious.In a normal rat liver tissue of TGF-β and α-SMA were significantly lower in model group and each treatment group, and there were significant differences, and the TGF-β and α-SMA in expression of liver tissue in model rats of TGF-β and α-SMA the highest. Conclusion: Oviductus ranae can effectively improve liver fibrosis rats induced by CC1₄ liver function.Oviductus ranae can reduce the expression of TGF-β₁ in liver tissue of hepatic fibrosis rats induced by CCl₄ in. This may be one of the mechanisms of Oviductus ranae in prevention and treatment of liver fibrosis. Even though both increased expression of TGF-β and α-SMA expression, is able to determine TGF-β and α-SMA for the intervention of liver TGF-β signal transduction pathway in liver fibrosis.

Abstract: According to adjustment of teaching program, integration of curriculum, compilation of teaching materials, open laboratory, we explore the integrated teaching Mode in Pharmaceutical Analysis. The results showed that this teaching way can not only stimulate the students interest in the learning, deepen their understanding on the knowledge, but also cultivate their creative thinking and research ability, and to some extent, improve the students' independent thinking, analysis and problem-solving abilities.

Abstract: In order to utilize the beneficial Indigenous Microbial in objective reservoir, active formula systems with various nutriment components were investigated^[. The results of performance evaluation showed that the activation, emulsification, viscosity break, and oil displacement performance of nutritional formula system were approved. Based on the field verification, the beneficial Indigenous Microbial in produced fluid of post treatment well array was activated, oil quality was improved, displacement efficiency was advanced, and oil production was increased.

Abstract: This study aimed to optimize liposome-mediatedtransfection conditions of pEGFP-N1 in FRT cells and to investigatewhether the optimized conditions were the optimal liposome-mediated transfectionconditions of the other vectors in FRT cells or not. The pEGFP-N1 were transfectedinto FRT cells, with the conditions of different cell confluence、ratio and quantity of vectors / liposome. At the sametime, pEGFP-N1-Aquaporin1、pEGFP-N1-Aquaporin3and pEGFP-N1-Aquaporin4 were transfected into FRT cells, respectively, withconditions of being same as pEGFP-N1. The inverted fluorescent microscopy was used to observe cytotoxicity and the expressionof EGFP in FRT cells. Transfection efficiency was measured by flow cytometry and cell viability was measured by trypanblue exclusion. The results showed that the expression of EGFP reachedthe highest at 36h after transfection. Flow cytometryand trypan blue exclusion tests showed when the cell confluence was 70%、the ratio and quantity of vectors / liposome was 1: 4(2.0ng: 8.0μL), pEGFP-N1 got higher transfection efficiency (46.97±0.32) % andcell viability (63.47±0.32) %. Under the same conditions, AQP1、AQP3 and AQP4 got the highest transfection efficiency and higher cell viability, too. The study would provide anexperimental evidence for efficient transfection of the other vectors in FRT cells.

Abstract: We assessed the effectiveness and mechanism of action of Pecan oil on learning and memory and NF-κB levels in the hippocampus of rats after Morris water maze (MWM test). Pecan oil (200,400 or 800 mg/kg/d) were administered by intragavage once daily for 14 consecutive days. The Morris water maze test was used to evaluate the ability of Pecan oil to increase learning and memory impairment. The levels of NF-κB in hippocampus of rats were detected by Westernblot after MWM test. Compared to untreated controls (P<0.01), MWM could be prolonged after Pecan oil treatment (P<0.05 for low and intermediate dose groups). Westernblot analysis showed that the protein expression of NF-κB was decreased in different concentration Pecan oil(P<0.05 and P<0.01, respectively). The results suggest that Pecan oil is effective in improving the learning and memory in rats, the mechanism of which may be related NF-κB expression decreasing.

Abstract: Abstract: To investigate whether juglone could inhibits the proliferation on human cervical cancer cells (HeLa) in vitro. Cells were divided into control group, different concentration (10μM, 20μM, 50μM, 100μM and200μM) juglone groups for different durations. The viability of HeLa cells was detected by methyl thiazolyl tetrazolium (MTT) assay. The morphology changes of HeLa cells were observed by inverted microscope .The results showed that the viability of HeLa cells was decreased and the cell morphology was changed in a dose-dependent manner after treatment different concentration juglone for 24h when compared with control group. The results suggest that Juglone may be effective for the treatment of HeLa cells.

Abstract: Abstract: Objective: To investigate whether the extract of Juglans mandshurica maxim could inhibits the apoptosis induced by endogenous H₂O₂ on Endothelial Cell （EVC-304）in vitro. METHODS: Cultured EVC-304 cells were incubated with 10mUGOX or with 10mUGOX and different concentrations (25μg/ mL, 50μg/ mL, 100μg/ mL) of the extract of Juglans mandshurica maxim for 24h.The proliferation of EVC-304cells was detected by methyl thiazolyl tetrazolium (MTT) assay. The early apoptotic percent was measured by flow cytometry (FCM). RESULTS: MTT results showed that the inhibition proliferation of EVC-304 cells induced by endogenous H₂O₂ could be inhibited by the extract of Juglans mandshurica maxim in a dose -dependent manner. FCM assay indicated that, after treatment on EVC-304 cells with endogenous H₂O₂, the early apoptotic percent was increased, but the apoptosis rate was decreased significantly when the extract of Juglans mandshurica maxim added. CONCLUSION: the extract of Juglans mandshurica maxim protected significantly the cell damage and cell apoptosis induced by endogenous H₂O₂.

Abstract: Sulfate reducing bacteria were screened from the soil of Anhe tailing pond in Baoji in China. 22 Sulfate reducing bacterial strains were isolatedand their tolerance ability to cadmium and sulfate reduction capability were determined by nephelometry and barium sulfate precipitation, respectively. One strain named STP2-1-5 with higher sulfate reducing capacity were isolated and identified by 16S rRNA sequencing. Then it was treated by using Dielectric Barrier Discharge (DBD) plasma mutagenesis and their mutants called STP2-1-5(2) and STP2-1-5(4) were obtained. The datum showed that their sulfate reduction capacity increased by 0.1855 and 0.4820 than original bacteria respectively. STP2-1-5(2) and STP2-1-5(4) might be used in the cadmium contaminated soil remediation.

Abstract: The aim of the study: This study was conducted to evaluate oxidative stress damage model caused by reactive oxygen species production was prepared by heating injury and protective effect of SCP on spermatozoa membrane structure and function in vitro. Materials and methods: mouse eupyrene sperm suspensions were prepared and were randomly divided into six groups. The control group was maintained with PBS. The positive medicine group was received PBS contained vitamin E (0.25 mg /ml) .The SCP group was then given SCP (0.2, 0.4 and 0.8 mg /ml) respectively. All the groups except control group were treated with using heat stress injury. Degree of injury of spermatozoa membrane lipid peroxidation was detected. Results: The vitality of total superoxide dismutase (T-SOD) was increased and the quality of malondialdehyde (MDA) was decreased in different degree with each concentration of SCP. The protective effect of 0.4 mg /ml and 0.8 mg /ml SCP on spermatozoa membrane was obviously surpass to positive medicine group. These findings indicate that SCP could be potentially useful for the treatment of oxidative stress damage in spermatozoa.