The Effects of Immobilization Conditions and Materials on the Desulfurization Performance of Immobilized UP-3

Abstract:

Immobilization of the bacterium Agrobacterium tumefaciens UP-3, which was isolated from soil and sewage in the Shengli Oilfield, was studied in this paper. The suitable culture condition of growth cells was determined. The effects of immobilization conditions and materials on the desulfurization performance of immobilized cells were investigated. The results showed that the immobilized cells with mixture of sodium alginate (SA) and polyvinyl alcohol (PVA) as the immobilized carrier had good biodesulfurization characteristics; The optimum operation immobilization conditions are 4° C, the concentration of SA being 3% (w), the concentration of PVA and SA being 7% (w), and the ratio of carriers (ml) and cells (g) being 20. When DBT addition is 2.7 mM, the DBT degradation rate of immbobilized cells is above 60%.

• Periodical: Advanced Materials Research (Volume 936)
• Pages: 740-745
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.936.740
• Online since: June 2014
• Authors: Ning Guo, Ying Fei Hou*, Ya Ya Gu, Chun Hu Li
• Keywords: Biodesulfurization, Immobilized Cells, Polyvinyl Alcohol, Sodium Alginate

References

[1] J. van der Geer, J.A.J. Hanraads, R.A. Lupton, The art of writing a scientific article, J. Sci. Commun. 163 (2000) 51-59.

[2] H.M. Chen, Retrospective review of annual meeting of NPRA and prospect on world petroleum refining technologies, Petroleum Refining Engineering. 9 (2001) 1-5.

[3] Y. Tanaka, T. Matsui, J. Konishi, et al. Isolation and characterization of a moderate thermophile, Mycobacterium phlei GTIS10, capable of dibenzothiophene desulfurization Applied Microbiology And Biotechnology, 59 (2002) 325-328.

DOI: 10.1007/s00253-002-1030-8

[4] J.H. Chang, Y.K. Chang, H. W Ryu, et al. Desulfurization of light gas oil in Immobilized-cell systems of Gordona sp. CYKS1 and Nocardia sp. CYKS2, FEMS Microbiol Letter, 182 (2000) 309-312.

DOI: 10.1111/j.1574-6968.2000.tb08913.x

[5] M. Naito, T. Kawamoto, K. Fujino et al, Long-term repeated biodesulfurization by immobilized Rhodococcus erthropollis KA2-5-1, Applied Microbiology And Biotechnology. 55 (2001) 374-378.

DOI: 10.1007/s002530000527

[6] K. Kirimura, T. Furuya, Y. Nishii, et al. Biodesulfurization of dibenzothiophene and its derivatives through the selective cleavage of carbon-sulfur bonds by a moderately thermophilic bacterium Bacillus subtilis WU-S2B, Journal Biosicence and Bioengineering, 3(2001).

DOI: 10.1016/s1389-1723(01)80131-6

[7] K. Kirimura, T. Furuya, Y. Nishii, Y. Ishii, et al. Thermophilic biodesulfurization of dibenzothiophene and its derivatives by Mycobacterium phlei WU-F1, FEMS Microbiology Letter. 9 (2001)129-133.

DOI: 10.1111/j.1574-6968.2001.tb10875.x

[8] J. Konishi, T. Onaka, Y. Ishii, M. Suzuki, et al. Demonstration of the carbon-sulfur bond targeted desulfurization of benzothiophene by thermophilic Paenibacillus sp. strain A11-2 capable of desulfurizing dibenzothiophene, Journal Biosicence and Bioengineering. 2(2001).

DOI: 10.1111/j.1574-6968.2000.tb09152.x

[9] Z. Z. Li. Biochemical Engineering Fundamentals, Chemistry Industry, Press Beijing, 1999, pp.104-107.

[10] X. M. Zhai, D. T. Li. Organic Micropolluted Source Water Treatment by Immobilized Cells. Environmental Science. 6 (2000) 80-84.

[11] J. L. Wang, H. Ch. Shi. Study and Advance on Polyvinyl Alcohol (PVA) as Entrapping Agents for Immobilizing microbial. Industrial Microbiology. 2 (1998) 35-39.