Encapsulation in Expanded Clay as an Alternative Protection for Bacteria in Bioconcrete-Influence on Compressive and Tensile Strength

Article Preview

Abstract:

This study explores the potential of self-healing concrete with bacteria encapsulated in calcium lactate and expanded clay (LECA) to enhance the durability and strength of concrete structures. The effect of encapsulating Lysinibacillus sphaericus bacteria in LECA on the mechanical properties of concrete was investigated, including compressive and tensile strength. Calcium lactate acts as a precursor and nutrient source for the biomineralization process through Microbially Induced Calcium Carbonate Precipitation (MICP). Experimental results demonstrate that concrete with bacteria encapsulated in LECA exhibits a significant increase in compressive strength compared to conventional concrete and concrete containing non-encapsulated bacteria. This increase is attributed to the protection provided by LECA to the bacteria and calcium lactate, promoting their self-healing activity and improving the concrete's ability to withstand loads. An increase in compressive load was observed for design DR-5 compared to DR-0 (control), with increments of 3.40%, 0.21%, and 6.92% on days 7, 14, and 28, respectively. However, challenges were identified regarding tensile strength, as design DR-5 was initially lower than design DR-0 by 24.25% and 19.51% on days 7 and 14, respectively. Nonetheless, on day 28, design DR-5 surpassed the control design by 1.45%. This study concludes that the encapsulation of bacteria in LECA, along with calcium lactate as a nutrient source, is a promising strategy for enhancing the performance of self-healing concrete, opening new avenues for research and applications in sustainable construction.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

125-130

Citation:

Online since:

October 2025

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2025 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] E. Tziviloglou, V. Wiktor, H. M. Jonkers, and E. Schlangen, "Bacteria-based self-healing concrete to increase liquid tightness of cracks", Construction and Building Materials, vol. 122, p.118–125, 2016.

DOI: 10.1016/j.conbuildmat.2016.06.080

Google Scholar

[2] N. Renne, P. Kara De Maeijer, B. Craeye, M. Buyle, and A. Audenaert, "Sustainable Assessment of Concrete Repairs through Life Cycle Assessment (LCA) and Life Cycle Cost Analysis (LCCA)", Infrastructures, vol. 7, no. 10, p.128, 2022.

DOI: 10.3390/infrastructures7100128

Google Scholar

[3] O. F. Arbeláez Pérez, K. A. Delgado Varela, and J. D. Castañeda Mena, "Effect of incorporation of cane bagasse ash on mechanical properties and carbon dioxide emissions of concrete containing waste glass", Bol. Soc. Esp. Ceram. Vidr., vol. 62, no. 5, p.443–451, 2023.

Google Scholar

[4] B. Lu, C. Shi y G. Hou, "Strength and microstructure of CO2 cured low-calcium clinker", Construction Building Mater., vol. 188, p.417–423, 2018.

DOI: 10.1016/j.conbuildmat.2018.08.134

Google Scholar

[5] A. Shukla et al., "Effects of Various Pseudomonas Bacteria Concentrations on the Strength and Durability Characteristics of Concrete", Buildings, vol. 12, no. 7, p.993, 2022.

DOI: 10.3390/buildings12070993

Google Scholar

[6] S. Mondal y A. Ghosh, "Spore-forming Bacillus subtilis vis-à-vis non-spore-forming Deinococcus radiodurans, a novel bacterium for self-healing of concrete structures: A comparative study", Construction Building Mater., vol. 266, p.121122, 2021.

DOI: 10.1016/j.conbuildmat.2020.121122

Google Scholar

[7] H. Amer Algaifi, S. Abu Bakar, A. Rahman Mohd. Sam, M. Ismail, A. Razin Zainal Abidin, S. Shahir, and W. Ali Hamood Altowayti, "Insight into the role of microbial calcium carbonate and the factors involved in self-healing concrete," Construction and Building Materials, vol. 254, Art. no. 119258, 2020.

DOI: 10.1016/j.conbuildmat.2020.119258

Google Scholar

[8] S. Girish, G. V. Lingaraj, A. Girish, and T. Soumya, "Feasibility Study on Biocomposite as a Self-Healer of Cracks," Journal of The Institution of Engineers (India): Series A, vol. 103, no. 1, p.227–238, 2022.

DOI: 10.1007/s40030-021-00562-9

Google Scholar

[9] M. Amran, A. M. Onaizi, R. Fediuk, N. I. Vatin, R. S. Muhammad Rashid, H. Abdelgader, and T. Ozbakkaloglu, "Self-Healing Concrete as a Prospective Construction Material: A Review," Materials, vol. 15, no. 9, 2022.

DOI: 10.3390/ma15093214

Google Scholar

[10] F. Althoey, O. Zaid, M. M. Arbili, R. Martínez-García, A. Alhamami, H. A. Shah, and A. M. Yosri, "Physical, strength, durability and microstructural analysis of self-healing concrete: A systematic review," Construction and Building Materials, 2023.

DOI: 10.1016/j.cscm.2022.e01730

Google Scholar

[11] S. Khodijah Chaerun, R. Syarif, and R. Kresna Wattimena, "Bacteria incorporated with calcium lactate pentahydrate to improve the mortar properties and self-healing occurrence," Scientific Reports, vol. 10, Art. no. 15858, 2020.

DOI: 10.1038/s41598-020-74127-4

Google Scholar

[12] S. Shivanshi, G. Chakraborti, K. S. Upadhyaya, and N. Kannan, "A study on bacterial self-healing concrete encapsulated in lightweight expanded clay aggregates," Materials Today: Proceedings, 2023.

DOI: 10.1016/j.matpr.2023.03.541

Google Scholar

[13] H. Amjad, M. A. Zeb, R. A. Khushnood, and N. Khan, "Impacts of biomimetic self-healing of Lysinibacillus boronitolerans immobilized through recycled fine and coarse brick aggregates in concrete," Journal of Building Engineering, vol. 76, Art. no. 107327, 2023.

DOI: 10.1016/j.jobe.2023.107327

Google Scholar