In Vivo Evaluation of Porous Hydroxyapatite/Poly D/L-lactide Composite for Bone Substitutes and Scaffolds

Abstract:

We had investigated the biocompatibility, osteoconductivity, and biodegradability of a porous composite of hydroxyapatite (HA) and poly-DL-lactide (PDLLA) implanted into rabbit femoral condyles. It showed excellent osteoconductivity and biodegradability as a bone substitute. Newly formed bones were remodeled, and materials were resorbed almost completely at 78weeks after implantation. In consideration of its biocompatibility and degradability, we investigated its potential for use as a cellular scaffold and evaluated its osteoinductive property. On implantation to the rat dorsal subcutaneous tissue loaded with syngeneic bone marrow cells, osteogenesis with enchondral ossification was seen both on and in the material at 3 weeks after implantation. This osteogenesis in the HA/PDLLA tended to get mature and newly formed bone tissues were found in the material by 6weeks. To investigate the osteoinductive property material itself has, we attempted to implant this porous composite material to extra-osseous canine dorsal muscle. At 2months, osteogenesis was seen in the pores of the material. It indicated the material induced osteogenesis with intramembranous ossification process. Therefore, HA/PDLLA might be a desirable material for bone substitutes and cellar scaffolds with osteoconductive and osteoinductive property.

• Periodical: Key Engineering Materials (Volumes 309-311)
• Pages: 1311-1314
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.309-311.1311
• Online since: May 2006
• Authors: Shin Hasegawa, Jiro Tamura, Masashi Neo, Shunsuke Fujibayashi, Koji Goto, Yasuo Shikinami, Kenshi Okazaki, Takashi Nakamura
• Keywords: Biodegradability, Composite, Hydroxyapatite (HAP), Osteoconductivity, Osteoinductivity, Poly(D,L-Lactide) (PDLLA), Porous

References

[1] J.X. Lu, A. Gallur, B. Flautre, K. Anselme, M. Descamps, B. Thierry and P. Hardouin: J Biomed Mater Res, 42. 357-367 (1998).

DOI: 10.1002/(sici)1097-4636(19981205)42:3<357::aid-jbm3>3.0.co;2-i

[2] B.S. Chang, C.K. Lee, K.S. Hong, H.J. Youn, H.S. Ryu, S.S. Chung and K.W. Park: Biomaterals, 21. 1291-1298(2000).

[3] K.U. Lewandrowski, J.D. Gresser, D.L. Wise and D.J. Trantlo: Biomaterals, 21. 757-764(2000).

[4] K.A. Hing, S.M. Best, K.E. Tanner, W. Bonfield and P.A. Revell: J Biomed Mater Res, 68A. 187-200(2004).

DOI: 10.1002/jbm.a.10050

[5] B. Flautre, M. Descamps, C. Delecourt, M.C. Blary and P. Hardouin: J. Mat. Sci.: Mat. Med. 12. 679-682(2001).

DOI: 10.1023/a:1011256107282

[6] M. Hasegawa,Y. Doi and A. Uchida: J Bone Joint Surg[Br], 85-B. 142-147(2003).

[7] N. Tamai, A. Myoui, T. Tomita, T. Nakase, J. Tanaka, T. Ochi, H. Yoshikawa: J Biomed Mater Res, 59. 110-117(2002).

DOI: 10.1002/jbm.1222

[8] R. Fujita, A. Yokoyama, T. Kawasaki and T. Kohgo: J Oral Maxillofac Surg, 61. 1045-1053(2003).

[9] T. Furukawa, Y. Matusue, T. Yasunaga, Y. Nakagawa, Y. Okada, Y. Shikinami, M. Okuno and T. Nakamura: J Biomed Mater Res, 50. 410-419(2000).

DOI: 10.1002/(sici)1097-4636(20000605)50:3<410::aid-jbm16>3.0.co;2-y

[10] J. Dong, H. Kojima, T. Uemura, M. Kikuchi, T. Tateishi and J. Tanaka: J Biomed Mater Res, 57. 208-216(2001).