Preparation of Porous Organically-Modified Silicate Hybrids for Cell Culture Matrix

Abstract:

Article Preview

Porous & rubbery organic-inorganic hybrids were synthesized from tetraethoxysilane (TEOS) and polydimethylsiloxane (PDMS) through a sol-gel route using sieved sucrose granules as a porogen. The porous hybrids with a high content of PDMS behaved like polymer sponge. The porosity was over 90% irrespective of the hybrid composition and the pore diameter ranged from 100 to 500 μm. In the three-dimensional cell culture, mammalian cells were well cultured in the porous hybrids. The present results indicate that the hybrids may be a promising scaffold for developing such functional culture methods.

Info:

Periodical:

Key Engineering Materials (Volumes 330-332)

Main Theme:

Edited by:

Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu

Pages:

1177-1180

Citation:

K. Tsuru et al., "Preparation of Porous Organically-Modified Silicate Hybrids for Cell Culture Matrix", Key Engineering Materials, Vols. 330-332, pp. 1177-1180, 2007

Online since:

February 2007

Export:

Price:

$38.00

[1] L. Ren, K. Tsuru, S. Hayakawa and A. Osaka: Biomaterials, Vol. 23 (2002), p.4765.

[2] Y. Shirosaki, K. Tsuru, S. Hayakawa, A. Osaka, M.A. Lopes, , M.H. Fernandes: Biomaterials, Vol. 26 (2005) p.485.

[3] T. Yabuta, E.P. Bescher, J.D. Mackenzie, S. Hayakawa and A. Osaka: J. Sol-Gel Sci. Technol., Vol. 26 (2003) p.1219.

[4] Y. Hu and J.D. Mackenzie: J. Mater. Sci. Vol. 27 (1992), p.4415.

[5] K. Tsuru, C. Ohtsuki, A. Osaka, T. Iwamoto and J.D. Mackenzie: J. Mater. Sci.: Mater. Med., Vol. 8 (1997), p.157.

[6] K. Tsuru, Y. Aburatani, T. Yabuta, S. Hayakawa and A. Osaka: J. Sol-Gel Sci. Technol., Vol. 21 (2001), p.89.

[7] K. Kataoka, R.J. Medina, M. Miyazaki, T. Yoshino, T. Makino, NH. Huh: Am. J. Pathol., Vol. 163 (2003) p.1227.

[8] P. Boukamp, R.T. Petrussecska, J. Hornung, A. Markham, N.E. Fusenig: J. Cell. Biol., Vol. 106 (1998), p.761.

[9] T. Yamane: Clin. Orthop., Vol. 199 (1985), p.261.

[10] K. Fukaya, S. Asahi, S. Nagamori, M. Sakaguchi, M. Namba: In Vitro Cell Dev Biol Anim , Vol. 37 (1991) , p.266.

[11] D.P. Aden, A. Fogel, S. Plotkin, I. Damjanov, B.B. Knowles: Nature, Vol. 282 (1979), p.615.

[12] T.H. Yang, H. Miyoshi, N. Ohshima: J. Biomed. Mater. Res., Vol. 55 (2001), p.379.

[13] S.F. Hulbert, J.S. Morrison and J.J. Klawitter: J. Biomed. Mater. Res., Vol. 6 (1972), p.347.

[14] T. J. Flatley, K.L. Lynch, M. Benson: Tissue Clin. Orthop., Vol. 179 (1983).

Fetching data from Crossref.
This may take some time to load.