Study of Protein Adsorption and Conformational Change on DLC and Ti Surfaces Using FTIR and Coomassie Brilliant Blue Protein Assay
The aim of present work was to study the interaction between human plasma protein-albumin (Alb) and immunoglobulin G (IgG) and the surfaces of two kinds of diamond-like carbon (DLC-A and DLC-B) and titanium (Ti) film. Fourier transform infrared spectroscopy (FTIR) was used to perform both quantity investigation and secondary structure analysis of above two proteins adsorbed on material surfaces. A modified Coomassie brilliant blue (CBB) protein assay was also used to study the amount of adsorbed proteins. The result of FTIR quantitative evaluation shows that the ratio of adsorbed Alb to IgG (RA/I) on three kinds of material surface has an order: DLC-A > DLC-B > Ti, which is coincide with the result from CBB protein assay. The result of secondary structure analysis shows that the conformation of Alb and IgG changes in a largest degree after adsorbed on Ti and a smallest degree on DLC-A surface. Both the results indicate that the anti-thrombogenicity of DLC-A seems to be the best and Ti is the worst.
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
H. Yan et al., "Study of Protein Adsorption and Conformational Change on DLC and Ti Surfaces Using FTIR and Coomassie Brilliant Blue Protein Assay", Key Engineering Materials, Vols. 330-332, pp. 893-896, 2007