A Novel Preconcentration Method for Microchip Based Electrophoresis and its Numerical Simulation

Abstract:

A novel preconcentration method for efficient DNA sample stacking during sample introduction and injection in microchip electrophoresis is presented in this paper. The method suggests that a pair of sample stacking electrodes is designed after the double-T section. A sample stacking electrical field can be established after sample introduction inside the double-T section, so that the sample stacking procedure can be controlled accurately and efficiently. In order to test this method’s validity, a numerical model is setup using COMSOL Multiphysics software. The simulation result shows that the peak DNA concentration can be increased up to 10 fold by this method. These results demonstrate the great potential for significantly enhance DNA sequencing speed and resolution in microchip electrophoresis.

• Periodical: Key Engineering Materials (Volume 503)
• Pages: 216-221
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.503.216
• Online since: February 2012
• Authors: Hao Yuan Cai, Xing Chen, Hui Li, Da Fu Cui
• Keywords: DNA Sequencing, Microchip Electrophoresis, Numerical Modeling, Sample Stacking

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