A Novel Unidirectional Porous Hydroxyapatite Cylinder Implanted in the Dorsal Muscles of Dogs Promotes Fibrous Tissue Vascularization and Invasion

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Abstract:

We recently synthesized a novel unidirectional porous hydroxyapatite (UDPHAp) material with a microstructure consisting of cross-sectional oval pores (diameter, 100-300 μm) . The unidirectional pores of UDPHAp are expected to facilitate the ingrowth of new tissues at sites of implantation. Here, we estimated the osteoinductive capacity of UDPHAp following its implantation in the dorsal muscles of dogs, and also investigated the affinity of UDPHAp for muscle and connective tissues. As a reference material, the HAp porous ceramic product Apaceram® (HOYA, Tokyo, Japan), which is commercially available in Japan and has a different microstructure from UDPHAp, was also used. A cylinder-shaped UDPHAp block was implanted in the dorsal muscles of two beagle dogs. At 1 and 2 years post-implantation, muscle and connective tissues had directly attached to UDPHAp at the upper and lower perforated surfaces. Histological assessment, revealed the direct invasion of fibrous tissues and small capillaries into the unidirectional pores of UDPHAp. Notably, no osseous tissue had formed within UDPHAp. Our findings suggest that the unidirectional pores of UDPHAp are advantageous for the vascularization and invasion of fibrous tissues. However, this unique structure does not contribute to osteoinductive capacity.

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