Microfluidic Amperometric Detection Sensor with Integrated Ag/AgCl Reference Electrode

Abstract:

In this paper, we presented a novel method for fabricating the integrated electrochemical sensor based on a microfluidic device. The device included: printed circuit board (PCB) substrate with vertical embedded electrodes; polymethyl methacrylate (PMMA) with micro structures; PMMA cover with amperometric detection sensor (ADS). The ADS employed three-electrode sensor system which was composed by two Au electrodes and an Ag/AgCl reference electrode. The polyvinyl chloride (PVC) membrane was used as ion-exchange medium of reference electrode and installed between Au electrodes. Then, the potential changes and the standardization oxidation-reduction experiments were executed to characterize the integrated reference electrode performance. Finally, the ADS system was tested by cyclic voltammetry (CV).

• Periodical: Key Engineering Materials (Volumes 645-646)
• Pages: 736-740
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.645-646.736
• Online since: May 2015
• Authors: He Zhang*, Xiao Wei Liu, Li Tian, Xiao Wei Han, Tong Dai
• Keywords: Composite Structure, Electro-Osmotic, Microfluidic, Vertical Embedded Electrodes

References

[1] Dykstra P H, Roy V, Byrd C, et al. Microfluidic electrochemical sensor array for characterizing protein interactions with various functionalized surfaces[J]. Analytical chemistry, 2011, 83(15): 5920-5927.

DOI: 10.1021/ac200835s

[2] Wang J N. A Microfluidic long-period fiber grating sensor platform for chloride ion concentration measurement[J]. Sensors, 2011, 11(9): 8550-8568.

DOI: 10.3390/s110908550

[3] Kim J, Elsnab J, Gehrke C, et al. Microfluidic integrated multi-walled carbon nanotube (MWCNT) sensor for electrochemical nucleic acid concentration measurement[J]. Sensors and Actuators B: Chemical, 2013, 185: 370-376.

DOI: 10.1016/j.snb.2013.05.018

[4] Cardenas-Valencia A M, Dlutowski J, Knighton S, et al. Aluminum-anode, silicon-based micro-cells for powering expendable MEMS and lab-on-a-chip devices[J]. Sensors and Actuators B: Chemical, 2007, 122(1): 328-336.

DOI: 10.1016/j.snb.2006.06.002

[5] Liu B, Rieck D, Van Wie B J, et al. Bilayer lipid membrane (BLM) based ion selective electrodes at the meso-, micro-, and nano-scales[J]. Biosensors and Bioelectronics, 2009, 24(7): 1843-1849.

DOI: 10.1016/j.bios.2008.09.019

[6] Ul Haque A, Rokkam M, De Carlo A R, et al. Design, Fabrication and Characterization of an In Silico Cell Physiology lab for Bio Sensing Applications[C]/Journal of Physics: Conference Series. IOP Publishing, 2006, 34(1): 740.

DOI: 10.1088/1742-6596/34/1/122

[7] Shinwari M W, Zhitomirsky D, Deen I A, et al. Microfabricated reference electrodes and their biosensing applications[J]. Sensors, 2010, 10(3): 1679-1715.

DOI: 10.3390/s100301679

[8] Polk B J, Stelzenmuller A, Mijares G, et al. Ag/AgCl microelectrodes with improved stability for microfluidics[J]. Sensors and Actuators B: Chemical, 2006, 114(1): 239-247.

DOI: 10.1016/j.snb.2005.03.121

[9] Chen Y P, Zhao Y, Chu J, et al. Fabrication and characterization of an innovative integrated solid-state microelectrode[J]. Electrochimica Acta, 2010, 55(20): 5984-5989.

DOI: 10.1016/j.electacta.2010.05.053

[10] Zhou J, Ren K, Zheng Y, et al. Fabrication of a microfluidic Ag/AgCl reference electrode and its application for portable and disposable electrochemical microchips[J]. Electrophoresis, 2010, 31(18): 3083-3089.

DOI: 10.1002/elps.201000113

[11] Zhang H, Liu X W, Tian L, et al. Organic Solvent Fumigation Bonding for Multi-Layer Poly (methyl Methacrylate) Microfluidic Device[J]. Key Engineering Materials, 2014, 609: 654-659.

DOI: 10.4028/www.scientific.net/kem.609-610.654