Investigation of Biodegradation Behavior of an Mg-1Ca Alloy during In Vivo Testing

Abstract:

In case of an orthopedic implant, it would be ideal that resorption to occur by biodegradation and bone remodeling. The main advantage of using resorbable orthopedic implants is eliminating the need for a new surgical procedure. The use of pure magnesium for orthopedic implants shows some drawbacks, which need to be considered and evaluated by in vitro and in vivo assays. One of the main problems encountered when pure Mg is used as biodegradable implant is represented by a high corrosion rate, faster than the rate of bone formation. The aim of this study is testing and evaluation of Mg-1Ca alloy from biocompatibility in vivo point of view. The purpose of in vivo test was to demonstrate good biocompatibility and lack of local and systemic toxicity of implants made by Mg-1Ca alloy. The study was conducted by implanting Mg-1Ca alloy parallelepiped shaped implants in the tibia of rabbits. In our tests related to Mg-1Ca alloy in vivo evaluation, there were no pathological increases in blood levels of Mg and Ca, or other elements, showing that it has no adversely affect to their metabolism. Also it shows a good bone integration, newly formed bone being adherent to the implant surface, with no tissue interposed between it and the bone. In conclusion, magnesium alloy Mg-1Ca represents a promising solution in orthopedic surgery, proving to be safe, with a high degree of biocompatibility and without toxic effects during in vivo testing.

• Periodical: Key Engineering Materials (Volume 752)
• Pages: 87-92
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.752.87
• Online since: August 2017
• Authors: Razvan Adam*, Horia Orban, Lavinia Dragomir, Claudia Milea, Iulian Antoniac, Adrian Barbilian
• Keywords: Biodegradable, Biodegradation, In Vivo, Mg-1Ca Alloy

References

[1] Witte F., Kaese V., Haferkamp H., Switzer E., Meyer-Lindenberg A., Wirth C.J., Windhagen H., In vivo corrosion of four magnesium alloys and the associated bone response, Biomaterials 26 (2005) 3557–3563.

DOI: 10.1016/j.biomaterials.2004.09.049

[2] Zakiyuddin A., Lee K., Effect of a small addition of zinc and manganese to Mg–Ca based alloys on degradation behavior in physiological media, Journal of Alloys and Compounds 629 (2015) 274–283.

DOI: 10.1016/j.jallcom.2014.12.181

[3] Adam R., Gheorghiu N., Orban H., Antoniac I.V., Barbilian A., Evolution toward new bioabsorbable material for osteosynthesis implants used in foot and ankle surgery, Archives of the Balkan Medical Union, 51(2) (2016) 190-198.

[4] Farraro K.F., Kim K.E., Woo S.L., Flowers J.R., McCullough M.B., Revolutionizing orthopaedic biomaterials: the potential of biodegradable and bioresorbable magnesium-based materials for functional tissue engineering, J. Biomech. 47 (2014).

DOI: 10.1016/j.jbiomech.2013.12.003

[5] Razavi M., Fathi M.H., Savabi O., Boroni M., A Review of degradation properties of Mg based biodegradable implants, Research and Reviews in Materials Science and Chemistry, 1(1) (2012) 15-58.

[6] Bita AI, Antoniac A, Cotrut C, Vasile E, Ciuca I, Niculescu M, Antoniac I, In vitro Degradation and Corrosion Evaluation of Mg-Ca Alloys for Biomedical Applications, JOAM, 18(3-4) (2016) 394-398.

DOI: 10.1080/01694243.2016.1171569

[7] Li N., Zheng Y., Novel Magnesium Alloys Developed for Biomedical Application: A Review, J. Mater. Sci. Technol. 29(6) (2013) 489e502.

[8] Li Z., Gu X., Lou S., Zheng Y., The development of binary Mg–Ca alloys for use as biodegradable materials within bone, Biomaterials 29(10) (2008) 1329–1344.

DOI: 10.1016/j.biomaterials.2007.12.021

[9] Kim W. C., Kim J.G., Lee J.Y., Seok H.K., Influence of Ca on the corrosion properties of magnesium for biomaterials, Materials Letters, 62 (25) (2008) 4146–4148.

DOI: 10.1016/j.matlet.2008.06.028

[10] Rau J.V., Antoniac I., Fosca M., De Bonis A., Blajan A.I., Cotrut C., Graziani V., Curcio M., Cricenti A., Niculescu M., Ortenzi M., Teghil R., Glass-ceramic coated Mg-Ca alloys for biomedical implant applications, Mater Sci Eng C Mater Biol Appl., 64 (2016).

DOI: 10.1016/j.msec.2016.03.100

[11] Mareci D., Bolat G., Izquierdo J., Crimu C., Munteanu C., Antoniac I., Souto R.M., Electrochemical characteristics of bioresorbable binary MgCa alloys in Ringer's solution: Revealing the impact of local pH distributions during in-vitro dissolution, Mater Sci Eng C Mater Biol Appl., 60 (2016).

DOI: 10.1016/j.msec.2015.11.069

[12] Feser K., Kietzmann M., Baumer W., Krause C., Bach F.W., Effects of degradable Mg-Ca alloys on dendritic cell function, J. Biomater. Appl. 25 (2011) 685–697.

DOI: 10.1177/0885328209360424

[13] Cha P.R., Han H.S., Yang G.F., Seok H.K., Biodegradability engineering of biodegradable Mg alloys: Tailoring the electrochemical properties and microstructure of constituent phases, Scientific Reports 3: 2367 (2013) 1-6.

DOI: 10.1038/srep02367

[14] Thomann M., Krause C., Bormann D., von der Hoh N., Windhagen H., Meyer-Lindenberg A., Comparison of the resorbable magnesium alloys LAE442 und MgCa0. 8 concerning their mechanical properties, their progress of degradation and the bone-implant contact after 12 months implantation duration in a rabbit model, Materialwiss Werksttech, 40 (1-2) (2009).

DOI: 10.1002/mawe.200800412