Metallic Film Modified Screen-Printed Carbon Electrode for Determination of 17α-Methyltestosterone

Abstract:

17α-methyltestosterone (MT) is a synthetic androgen. It is used widely for inducing an all-male population of Nile tilapia (Oreochromis niloticus). In this work, the detection of MT was conducted using screen-printed carbon electrodes (SPCE). These were a bare electrode, a bismuth modified electrode (Bi-SPCE) and an antimony modified electrode (Sb-SPCE). The successful electrode modification was confirmed by scanning electron microscopy. The electroanalytical performance of the SPCE modified electrodes for MT detection was examined by cyclic voltammetry. The highest active surface area of 1.073x10^-4 cm² was obtained on Sb-SPCE. This indicates that Sb-SPCE can enhance the sensitivity of MT detection better than the bare-SPCE and the Bi-SPCE. The Sb-SPCE showed a linear response for MT concentrations ranging from 2 to 8 mg.L^-1. The sensitivity obtained from the slope of a calibration curve was -0.452 mA.mol^-1.L^-1 in a Britton-Robinson buffer pH 4.0 containing Sb 16 mg.L^-1 with deposition potential and deposition time of 1 V and 90 seconds, respectively. A linear relationship between the square root of the scan rate and the peak current revealed that mass transfer of MT to the electrode was driven by a diffusion mechanism. The limit of detection was found to be 1 mg.L^-1.

• Periodical: Key Engineering Materials (Volume 824)
• Pages: 182-189
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.824.182
• Online since: October 2019
• Authors: Chim Math, Wijitar Dungchai, Sudtida Pliankarom Thanasupsin*
• Keywords: 17 α-Methyltestosterone, Metallic Film, Screen-Printed Carbon Electrode (SPCE)

References

[1] Z.G. Shen, Q.X. Fan, W. Yang, Y.L. Zhang, H.P. Wang, Effects of 17 α-methyltestosterone and aromatase inhibitor letrozole on sex reversal, gonadal structure, and growth in yellow catfish Peltobagrus fulvidaco, Biol. Bull. 228 (2015) 108 –117.

DOI: 10.1086/bblv228n2p108

[2] J. Gao, S. Liu, Y. Zhang, Y. Yang, C. Yuan, S. Chen, Z. Wang, Effects of 17 α-methyltestosterone on transcriptome, gonadal histology and sex steroid hormones in rare minnow (Gobiocypris rarus), Comp. Biochem. Physiol., Part D: Genomics Proteomics. 15 (2015) 20-27.

DOI: 10.1016/j.cbd.2015.05.001

[3] M. Seki, H. Yokota, H. Matsubara, M. Maeda, H. Todokoro, K. Kobayashi, Fish full life-cycle testing for androgen methyltestosterone on medaka (Oryzias Latipes), Environ. Toxicol. Chem. 23 (2004) 774-81.

DOI: 10.1897/03-26

[4] Q. Zhong, Y. Hu, G.Li, A novel protocol for molecularly imprinted polymer filaments online coupled to GC-MS for the determination of androgenic steroids in urine, J. Sep. Sci. 36 (2013) 3903-3910.

DOI: 10.1002/jssc.201300874

[5] I. R. Barbosa, S. Lopes, R. Oliveira, I. Domingues, A.M.V.M. Soares, A.J.A. Nogueira, Determination of 17 α-methyltestosterone in freshwater samples of tilapia farming by high-performance liquid chromatography, Am. J. Anal. Chem. 04 (2013) 207-211.

DOI: 10.4236/ajac.2013.44026

[6] P.S. Chu, M. Lopez, S. Serfling, C. Gieseker, R. Reimschuessel, Determination of 17α-methyltestosterone in muscle tissues of tilapia, rainbow trout, and salmon using liquid chromatography-tandem mass spectrometry, J. Agric. Food Chem. 54 (2006) 3193-3198.

DOI: 10.1021/jf052701r

[7] H. Shengshui, C. Zilin, Z. Tao, Adsorptive stripping voltammetry of testosterone propionate in pharmaceutical Preparations, J. Anal. Chem. 346 (1993) 1008-1010.

DOI: 10.1007/bf00322768

[8] R.N. Goyal, V.K. Gupta, S. Chatterjee, Electrochemical investigations of corticosteroid isomers-testosterone and epitestosterone and their simultaneous determination in human urine, Anal. Chem. Acta. 657 (2010) 147-153.

DOI: 10.1016/j.aca.2009.10.035

[9] A. Levent, A. Altun, S. Taş, Y. Yardım, Z. Şentürk, Voltammetric behavior of testosterone on bismuth film electrode: highly sensitive determination in pharmaceuticals and human urine by square-wave adsorptive stripping voltammetry, Electroanalysis. 27 (2015) 1219-1228.

DOI: 10.1002/elan.201400627

[10] L. Miranda, A. Galli, S.P. Quináia, Endocrine interfering in natural waters: voltammetric determination of 17α-methyltestosterone, Rev. Virtual Quim. 6 (2014).

DOI: 10.5935/1984-6835.20140029

[11] O.D. Renedo, M.A. Alonso-Lomillo, M.J. Martinez, Recent developments in the field of screen-printed electrodes and their related applications, Talanta. 73 (2007) 202-219.

DOI: 10.1016/j.talanta.2007.03.050

[12] M. Li, D.W. Li, G. Xiu, Y.T. Long, Applications of screen-printed electrodes in current environmental analysis, Curr. Opin. Electrochem. 3 (2017) 137-143.

[13] M. Li, Y.T. Li, D.W. Li, Y.T. Long, Recent developments and applications of screen-printed electrodes in environmental assays-a review, Anal. Chem. Acta. 734 (2012) 31-44.

DOI: 10.1016/j.aca.2012.05.018

[14] M. Eguilaz, M. Moreno-Guzman, S. Campuzano, A. Gonzalez-Cortes, P. Yanez-Sedeno, J.M. Pingarron, An electrochemical immunosensor for testosterone using functionalized magnetic beads and screen-printed carbon electrodes, Biosens. Bioelectron. 26 (2010) 517-522.

DOI: 10.1016/j.bios.2010.07.060

[15] N. Serrano, J.M. Díaz-Cruz, C. Ariño, M. Esteban, Antimony-based electrodes for analytical determinations, TrAC, Trends Anal. Chem. 77 (2016) 203-213.

DOI: 10.1016/j.trac.2016.01.011

[16] C. Chen, X. Niu, Y. Chai, H. Zhao, M. Lan, Y. Zhu, G. Wei, Determination of lead(II) using screen-printed bismuth-antimony film electrode, Electroanalysis. 25 (2013) 1446-1452.

DOI: 10.1002/elan.201200625

[17] M. Libansky, J. Zima, J. Barek, A. Reznickova, V. Svorcik, H. Dejmkova, Basic electrochemical properties of sputtered gold film electrodes, Electrochim. Acta. 251 (2017) 452-460.

DOI: 10.1016/j.electacta.2017.08.048

[18] N.P. Shetti, D.S. Nayak, S.J. Malode, R.M. Kulkarni, An electrochemical sensor for clozapine at ruthenium doped TiO2 nanoparticles modified electrode, Sens. Actuators, B. 247 (2017) 858-867.

DOI: 10.1016/j.snb.2017.03.102