Fabrication of Low Temperature Carbonated Hydroxyapatite Porous Scaffolds for Bone Tissue Engineering Applications

Abstract:

Three-dimensional (3D) porous carbonated hydroxyapatite (CHA) scaffolds were successfully prepared using polyurethane (PU) replication technique. Two sets of porous scaffolds were prepared using as-synthesized and as-calcined CHA powder as the main component of the slurry. The effect of the condition of starting material was investigated in terms of structure, phase purity, crystallinity and morphology of the fabricated porous scaffolds. Regardless of the condition of starting material used, the porous scaffolds fabricated was single phase B-type CHA and free of secondary phases. Interestingly, scaffolds made of as-calcined CHA powder (SC scaffolds) showed a smoother surface and more solidified struts when compared to as-synthesized CHA powder (SA scaffolds). This is attributed to the state of semi-crystalline phase of the as-calcined powder being amorphous phase. SC scaffold was found to be better scaffold with respect to handling, compaction strength and microstructure with better strut properties.

• Periodical: Key Engineering Materials (Volume 829)
• Pages: 167-172
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.829.167
• Online since: December 2019
• Authors: Muhammad Syazwan Mohd Noor, Ahmad Fauzi Mohd Noor, Yanny Marliana Baba Ismail*
• Keywords: Bone Tissue Engineering, Carbonated Hydroxyapatite, Porous Scaffold

References

[1] A. Kataruka, K. Mendu, O. Okeoghene, J. Puthuvelil, and A.-T. Akono, Microscopic assessment of bone toughness using scratch tests, Bone Rep. 6 (2017) 17–25.

DOI: 10.1016/j.bonr.2016.12.001

[2] S. Bose, G. Fielding, S. Tarafder, and A. Bandyopadhyay, Understanding of dopant-induced osteogenesis and angiogenesis in calcium phosphate ceramics, Trends Biotechnol. 31 10 (2013) 594–605.

DOI: 10.1016/j.tibtech.2013.06.005

[3] E. Boanini, M. Gazzano, and A. Bigi, Ionic substitutions in calcium phosphates synthesized at low temperature, Acta Biomater. 6 6 (2010) 1882–1894.

DOI: 10.1016/j.actbio.2009.12.041

[4] S. Dasgupta, S. S. Banerjee, A. Bandyopadhyay, and S. Bose, Zn- and Mg-doped hydroxyapatite nanoparticles for controlled release of protein, Langmuir. 26 7 (2010) 4958–4964.

DOI: 10.1021/la903617e

[5] S. H. Min, H. H. Jin, H. Y. Park, I. M. Park, H. C. Park, and S. Y. Yoon, Preparation of porous hydroxyapatite scaffolds for bone tissue engineering, Mater. Sci. Forum. 510–511 (2006) 754–757.

DOI: 10.4028/www.scientific.net/msf.510-511.754

[6] L. T. Bang, B. D. Long, and R. Othman, Carbonate hydroxyapatite and silicon-substituted carbonate hydroxyapatite: Synthesis, mechanical properties, and solubility evaluations, Sci. World J. 2014 (2014).

DOI: 10.1155/2014/969876

[7] R. Othman, Z. Mustafa, C. Loon, and A. Noor, Effect of calcium precursors and pH on the Precipitation of carbonated hydroxyapatite, Procedia Chem. 19 (2016) 539–545.

DOI: 10.1016/j.proche.2016.03.050

[8] D. Bellucci, A. Sola, and V. Cannillo, A revised replication method for bioceramic scaffolds, Bioceram. Dev. Appl. 1 (2011) 1–8.

DOI: 10.4303/bda/d110401

[9] Y. Liu, J. Lim, and S.H. Teoh, Review: Development of clinically relevant scaffolds for vascularised bone tissue engineering, Biotechnol. Adv. 31 5 (2013) 688–705.

DOI: 10.1016/j.biotechadv.2012.10.003

[10] S. Tajbakhsh and F. Hajiali, A comprehensive study on the fabrication and properties of biocomposites of poly(lactic acid)/ceramics for bone tissue engineering, Mater. Sci. Eng. C. 70 (2016) 897–912.

DOI: 10.1016/j.msec.2016.09.008

[11] A.C.B.M. Fook, A.H. Aparecida, T.B. Fidéles, R.C. Costa, and M.V.L. Fook, Porous hydroxyapatite scaffolds by polymer sponge method, Key Eng. Mater. 396–398 (2009) 703–706.

DOI: 10.4028/www.scientific.net/kem.396-398.703

[12] N. Monmaturapoj and C. Yatongchai, Influence of preparation method on hydroxyapatite porous scaffolds, Bull. Mater. Sci. 34 7 (2011) 1733–1737.

DOI: 10.1007/s12034-011-0384-x

[13] M. Bricha, Y. Belmamouni, E.M. Essassi, J.M.F. Ferreira, and K. El Mabrouk, Surfactant-assisted hydrothermal synthesis of hydroxyapatite nanopowders, J. Nanosci. Nanotechnol. 12 10 (2012) 8042–8049.

DOI: 10.1166/jnn.2012.6664

[14] Y.M. Baba Ismail, I. Wimpenny, O. Bretcanu, K. Dalgarno, and A.J. El Haj, Development of multisubstituted hydroxyapatite nanopowders as biomedical materials for bone tissue engineering applications, J. Biomed. Mater. Res. - Part A. (2017) 1–11.

DOI: 10.1002/jbm.a.36038

[15] E.S. Kovaleva, M.P. Shabanov, V.I. Putlayev, Y.Y. Filippov, Y.D. Tretyakov, and V.K. Ivanov, Carbonated hydroxyapatite nanopowders for preparation of bioresorbable materials, Materwiss. Werksttech. 39 11 (2008) 822–829.

DOI: 10.1002/mawe.200800383

[16] E. Landi, G. Celotti, G. Logroscino, and A. Tampieri, Carbonated hydroxyapatite as bone substitute, J. Eur. Ceram. Soc. 23 15 (2003) 2931–2937.

DOI: 10.1016/s0955-2219(03)00304-2

[17] Y.M. Baba Ismail and A.F. Mohd Noor, Effect of a novel approach of sintering on physical properties of carbonated hydroxyapatite, J. Mater. Sci. Eng. B. 1 2 (2011) 157–163.

[18] J.P. Lafon, E. Champion, and D. Bernache-assollant, Processing of AB-type carbonated hydroxyapatite ceramics with controlled composition, J. Eur. Ceram. Soc. 28 (2008) 139–147.

DOI: 10.1016/j.jeurceramsoc.2007.06.009

[19] Y.J. Guo, T. Long, W. Chen, C.Q. Ning, Z.A. Zhu, and Y.P. Guo, Bactericidal property and biocompatibility of gentamicin-loaded mesoporous carbonated hydroxyapatite microspheres, Mater. Sci. Eng. C. 33 7(2013) 3583–3591.

DOI: 10.1016/j.msec.2013.04.021