Preparation and Application of Immobilized Cells on Amino Acid-Modified Magnetic Nanoparticles

Abstract:

The magnetic ferroferric oxide nanoparticles were modified with L-lysine to prepare immobilized carriers(Fe₃O₄-Lys), which was applied to the immobilization of Saccharomyces cerevisiae CGMCC No. 2230. The optimal immobilization conditions for preparation of Fe₃O₄-Lys-Cells were Fe₃O₄-Lys 0.01 g, cell dry weight 3 g, pH 7, 3 h, 30 °C. Ethyl R-4-chloro-3-hydroxybutyrate was gained by asymmetric reduction of ethyl-4-chlorooxobutanoate with Fe₃O₄-Lys-Cells as catalysis. 0.672 mol/L COBE can be converted completely in 40 h in the shaker, while only 24 h in the alternating magnetic field. The conversion and enantiomeric excess(e.e.) of (R)-CHBE reached 100% and more than 99.9%, respectively.

• Periodical: Materials Science Forum (Volume 1003)
• Pages: 150-155
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.1003.150
• Online since: July 2020
• Authors: Yuan Lu, Hong Qian Dai, Zhi Min Ou*
• Keywords: Amino Acid, Asymmetric Reduction, Ethyl R-4-Chloro-3-Hydroxybutyrate, Immobilized Cell, Magnetic Nanoparticles

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