Antibacterial Enhancement of 3D Printed Dental Resin Photopolymer Using Titanium Dioxide Nanoparticles

Article Preview

Abstract:

This study aims to evaluate the antibacterial properties of dental resin photopolymer (DRP) specimens modified with additive titanium dioxide (TiO2) nanoparticles using stereolithography 3D printing technology. TiO2 known for its excellent biocompatibility, making it a promising additive for enhancing bacterial resistance. Specimens were fabricated with varying compositions of TiO2 and characterized for surface morphology using Scanning Electron Microscopy (SEM). Antibacterial activity was assessed against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative) using Kirby-Bauer disc diffusion method. SEM analysis revealed that TiO2 particles were relatively well-dispersed on the matrix surface. Antibacterial testing showed the formation of inhibition zones, particularly in sample with composition 5%wt TiO2, indicating increasing antibacterial performance. The activity was more pronounced against S. aureus, attributed to its less complex cell wall structure and more susceptible to reactive oxygen species (ROS) generated by TiO2 photocatalytic conditions. These findings suggest that TiO2-modified DRP has strong potential as an antimicrobial dental restorative material fabricated through SLA-based 3D printing.

You might also be interested in these eBooks

Info:

Periodical:

Materials Science Forum (Volume 1189)

Pages:

53-59

Citation:

Online since:

May 2026

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2026 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] W. Harwijayanti, U. Ubaidillah, and J. Triyono, "Physicochemical Characterization and Antibacterial Activity of Titanium/Shellac-Coated Hydroxyapatite Composites," Coatings, vol. 12, no. 5, 2022.

DOI: 10.3390/coatings12050680

Google Scholar

[2] Rochmadi, W. Harwijayanti, U. Ubaidillah, J. Triyono, K. Diharjo, and P. Utomo, "The role of binder in the manufacturing of bone implants: A review," in AIP Conference Proceedings, 2023, vol. 2727, p.030011.

DOI: 10.1063/5.0142047

Google Scholar

[3] T. ten Brink, F. Damanik, J. I. Rotmans, and L. Moroni, "Unraveling and Harnessing the Immune Response at the Cell–Biomaterial Interface for Tissue Engineering Purposes," Adv. Healthc. Mater., vol. 13, no. 17, Jul. 2024.

DOI: 10.1002/adhm.202301939

Google Scholar

[4] S. J. Ahmed, N. J. Abdulridha, A. J. Al-Obaidi, H. Dalfi, and A. Alomarah, "Investigating the Mechanical, Physical and Biological Properties of PMMA/TiO2 Composites with Nanoclay for Denture Applications," Eng. Trans., vol. 73, no. 1, p.29–43, 2025.

Google Scholar

[5] İ. Aktitiz, K. Aydın, and A. Topcu, "Characterization of TiO2 Nanoparticle–Reinforced Polymer Nanocomposite Materials Printed by Stereolithography Method," J. Mater. Eng. Perform., vol. 30, no. 7, p.4975–4980, Jul. 2021.

DOI: 10.1007/s11665-021-05574-x

Google Scholar

[6] I. S. O. Barbosa, Y. A. Manrique, D. Paiva, J. L. Faria, R. J. Santos, and C. G. Silva, "Efficient photocatalytic reactors via 3D printing: SLA fabrication and TiO 2 hybrid materials," RSC Adv., vol. 15, no. 4, p.2275–2286, 2025.

DOI: 10.1039/D4RA07121B

Google Scholar

[7] E. E. Totu, A. C. Nechifor, G. Nechifor, H. Y. Aboul-Enein, and C. M. Cristache, "Poly(methyl methacrylate) with TiO 2 nanoparticles inclusion for stereolitographic complete denture manufacturing − the fututre in dental care for elderly edentulous patients?," J. Dent., vol. 59, p.68–77, Apr. 2017.

DOI: 10.1016/j.jdent.2017.02.012

Google Scholar

[8] M. Alamgir, A. Mallick, G. C. Nayak, and S. K. Tiwari, "Development of PMMA/TiO2 nanocomposites as excellent dental materials," J. Mech. Sci. Technol., vol. 33, no. 10, p.4755–4760, Oct. 2019.

DOI: 10.1007/s12206-019-0916-7

Google Scholar

[9] M. M. Gad and R. Abualsaud, "Behavior of PMMA Denture Base Materials Containing Titanium Dioxide Nanoparticles: A Literature Review," Int. J. Biomater., vol. 2019, p.1–14, Jan. 2019.

DOI: 10.1155/2019/6190610

Google Scholar

[10] G. Venkatesh Anehosur and R. D. Kulkarni, "Synthesis and Determination of Antimicrobial Activity of Visible Light Activated TiO2 Nanoparticles with Polymethyl Methacrylate Denture Base Resin Against Staphylococcus Aureus," J. Gerontol. Geriatr. Res., vol. 01, no. 01, p.1–8, 2012.

DOI: 10.4172/2167-7182.1000103

Google Scholar

[11] C. Billings, C. Cai, and Y. Liu, "Utilization of Antibacterial Nanoparticles in Photocurable Additive Manufacturing of Advanced Composites for Improved Public Health," Polymers (Basel)., vol. 13, no. 16, p.2616, Aug. 2021.

DOI: 10.3390/polym13162616

Google Scholar

[12] ISO, "Biological evaluation of medical devices," in ISO 10993-5:2009, vol. 3, no. 1, 2009, p.1–42.

Google Scholar

[13] Nasikhudin, M. Diantoro, A. Kusumaatmaja, and K. Triyana, "Study on Photocatalytic Properties of TiO 2 Nanoparticle in various pH condition," J. Phys. Conf. Ser., vol. 1011, no. 1, p.012069, Apr. 2018.

DOI: 10.1088/1742-6596/1011/1/012069

Google Scholar

[14] R. Krzosa, Ł. Makowski, W. Orciuch, G. Özcan-Taşkın, R. Adamek, and M. Wojasiński, "Characterization of structures and properties of TiO2 powders," Powder Technol., vol. 421, p.118437, May 2023.

DOI: 10.1016/j.powtec.2023.118437

Google Scholar

[15] I. De Pasquale et al., "Photocatalytic TiO2-Based Nanostructured Materials for Microbial Inactivation," Catalysts, vol. 10, no. 12, p.1382, Nov. 2020.

DOI: 10.3390/catal10121382

Google Scholar