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Fabrication of an Enzymatic Biosensor Based on Gold Nanoparticles Modified Electrochemical Transducer for the Detection of Organophosphorus Compounds

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Abstract:

A facile and sensitive enzyme based electrochemical transducer has been fabricated for the detection of organophosporus compounds. The enzyme, acetylcholinesterase, was covalently immobilized on gold nanoparticles deposited electrochemically over screen printed carbon working electrode. The electrodes were characterized by scanning electron microscopy, atomic force microscopy and electrochemical methods. The enzyme-substrate reactions and sensing studies were carried out at room temperature by cyclic voltammetry. The developed biosensor gave optimum response within 25 sec. for a substrate (acetylthiocholine) concentration of 0.0699 mM at pH. The electrode showed a linear response in the range between 0.2 and 1 ppb, and the detection limit was determined to be 0.6 ppb. Moreover the biosensor exhibited good reusability and stability thus, making it a promising tool for on-field detection of organophosphorus compounds.

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Nano Hybrids and Composites Vol. 12 View Preview

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Periodical:

Nano Hybrids and Composites (Volume 12)

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67-73

DOI:

https://doi.org/10.4028/www.scientific.net/NHC.12.67

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Online since:

November 2016

Authors:

Rini Grace, Keerthana Sundar, N. Mohan, D. Selvakumar*, N.S. Kumar

Keywords:

Acetylcholinestrase, Electrochemical Biosensor, Gold Nanoparticles, Organophosphorus Compounds

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© 2017 Trans Tech Publications Ltd. All Rights Reserved

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