On-Chip Fabrication of None-Dead-Volume Microtips for ESI-MS Applications
This paper proposes a novel method to on-chip fabricate a none-dead-volume microtip for ESI-MS applications. The microfluidic chip and ESI tip are fabricated in low-cost plastic based materials using a simple and rapid fabrication process. A constant-speed-pulling method is developed to fabricate the ESI tip by pulling mixed PMMA glue using a 30-μm stainless wire through the pre-formed microfluidic channel. The equilibrium of surface tension of PMMA glue will result in a sharp tip after curing. A highly uniform micro-tip can be formed directly at the outlet of the microfluidic channel with minimum dead-volume zone. Detection of caffeine, myoglobin, lysozyme and cytochrome C biosamples confirms the microchip device can be used for high resolution ESI-MS applications.
Chunli BAI, Sishen XIE, Xing ZHU
C. H. Lin et al., "On-Chip Fabrication of None-Dead-Volume Microtips for ESI-MS Applications", Solid State Phenomena, Vols. 121-123, pp. 611-614, 2007