Functional self-assembled structure with well-defined shapes and dimensions are of great current interest. Porphyrins are attractive building manifold patterning for these structures because of their electronic, optical, and catalytic properties. In this paper, we report a novel technique for the assembly patterning of polymers. An Electrospinning process was used to create patterning with diameter ranging from 3 μm to 4 μm and length of up to several hundred micrometers in the presence of Zinc Porphyrin. Scanning electron microscopy (SEM), UV- visible spectra and EDX spectra were used to characterize the patterning. The results indicated that the patterning depended on the concentration of Porphyrin and the operational conditions.