Efficient Evaluation Method of Cell’s Viability Using Dielectrophoresis

Ryosuke Komai; Keisyu Aritoshi; Kozo Taguchi

doi:10.4028/www.scientific.net/AMM.870.375

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 870Efficient Evaluation Method of Cell’s Viability...

Efficient Evaluation Method of Cell’s Viability Using Dielectrophoresis

Abstract:

We used Dielectrophoresis (DEP) to discover healthy cells and estimate the cells activity and viability. In the first experiment, we mixed dead cells with methylene blue solution. We applied voltage of 4V, frequency of 10 kHz and 15 MHz to the electrode [1], and observed the movement of the cells. This experiment proved that DEP could also be used to judge cell’s viability. In the next experiment, we used viable cells and raised water temperature rapidly to apply damage. Temperature of water was fixed at 30 degrees, 40 degrees, 50degrees, 60degrees, and 70 degrees. And we observed the cells movement while changing the frequency. We mixed methylene blue solution with the sample to confirm whether the yeast cells were dead or alive when water temperature was from 50 degrees to 70 degrees. These experiments proved that it was possible to measure the activity of cells by changing the DEP frequency.

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Periodical:

Applied Mechanics and Materials (Volume 870)

Pages:

375-379

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.870.375

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Online since:

September 2017

Authors:

Ryosuke Komai, Keisyu Aritoshi, Kozo Taguchi

Keywords:

Cell Activity, Cell Viability, Dielectrophoresis (DEP)

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References

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