The hydroxamate-based artificial siderophore that contains terminated-amine group was newly synthesized. This artificial siderophore formed stable complex with FeIII ion and high bio-activities. The FeIII-artificial siderophore complex was attached onto the glass substrate surface by stepwise self-assembling method. The obtained artificial siderophore-modified glass substrate adsorbed the Gram-positive bacterium Microbacterium. flavescens. The Gram-negative bacterium Escherichia. coli was not adsorbed. The artificial siderophore-modified glass substrates indicated the selective adsorption of microorganisms.