NFES is a new and simple way to realize precision-positioning of nanofiber. A model on NFES nanofiber movement is built to analyze the effects of the existed nanofibers which have been collected on the substrate, on the nanofiber’s dropping movement. During electrospinning nanofiber is affected by the electric field force, Coulomb repulsive force, air resistance force gravity and so on. The influence of parameters on the deposition behavior of as-spun nanofiber is discussed. The simulation results show that (i) with charge density increasing, the final spacing between mass center of nanofibers A and B (FSAB) increases and the movement distance of center-of-mass of nanofiber B (MDB) decreases first and then increases; (ii) FSAB increases with applied voltage, but decreased in narrow range with concentration of PEO increasing; (iii) FSAB decreased with the initial spacing between mass center of nanofibers A and B (ISAB) increasing, and then it increases after reaching the minimum. So does ISAB to DMB. This simulation model would improve the controlling of nanofiber in NFES.